天然和人为来源颗粒物对大肠杆菌B906生长指标及对抗生素敏感性的影响

Q4 Biochemistry, Genetics and Molecular Biology Mikrobiolohichnyi zhurnal Pub Date : 2023-08-16 DOI:10.15407/microbiolj85.04.034
М.Yu. Korbush, Т.M. Serhiichuk, Y. Yumyna, T.O. Borisova, G. Tolstanova
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引用次数: 0

摘要

颗粒物(PM)是污染空气的主要成分之一,可促进胃肠道疾病的发展,并改变肠道微生物群的组成及其代谢特性。目标。本研究主要分析了棉杨木(PMC)和医用口罩(PMM)燃烧产生的不同浓度的PM对乳糖阳性大肠杆菌B906生长强度、生物膜形成能力和抗生素敏感性的影响。方法。在MPA培养基中接种浓度为105 CFU/mL的大肠杆菌B906培养物,然后分别加入浓度为18、36、72 μg/mL的PMC和PMM。通过分光光度计测量72h的光密度来确定生长强度。为了确定活细胞的数量及其发酵乳糖的能力,在Endo培养基上进行播种。用染色和解吸法测定了聚苯乙烯板上的生物膜形成能力。采用圆盘扩散法测定抗生素敏感性(氨苄西林、左旋霉素、美罗培南、诺氟沙星、头孢曲松),培养24、48、72 h。结果。PMC和PMM对大肠杆菌B906的生长均有抑制作用,在浓度为72 μg/mL时,生物量几乎没有增加。PMC培养基在浓度为18 μg/mL和36 μg/mL时,活性细胞数比对照减少1-2个数量级,在浓度为72 μg/mL时,活性细胞数比对照减少6个数量级。在此浓度下,48和72 h未见生长。PMM具有抑菌作用:在Endo培养基上,18 μg/mL和36 μg/mL的浓度下,24 ~ 72 h的活细胞数量减少1 ~ 2个数量级,72 μg/mL的浓度下,活细胞数量减少3 ~ 4个数量级。培养48 h时,PMC浓度为18 μg/mL和36 μg/mL时促进生物膜的形成,浓度为72 μg/mL时抑制生物膜的形成。相反,PMM在所有浓度下都降低了生物膜密度。两种类型的PM均诱导氨苄西林耐药,但对PMM的作用更强,并导致对诺氟沙星耐药。结论。本研究表明,PMC和PMM对乳糖阳性大肠杆菌B906有直接影响,表现为中、高浓度(36 μg/mL和72 μg/mL)下生长强度降低,酶活性降低,生物膜形成增强,对氨苄西林、头孢曲松和诺氟沙星产生耐药性。
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Effect of Particulate Matter of Natural and Anthropogenic Origin on Growth Indicators and Sensitivity to Antibiotics of Escherichia coli B906
Particulate matter (PM), which is among the main components of polluted air, can contribute to the development of gastrointestinal diseases and alter the composition of gut microbiota and its metabolic properties. Objective. The study focuses on analyzing the influence of different concentrations of PM derived from the combustion of cottonwood (PMC) and medical masks (PMM) on the growth intensity, biofilm formation capability, and antibiotic susceptibility of lactose-positive Escherichia coli strain B906. Methods. The MPA medium was inoculated with a culture of E. coli B906 at a concentration of 105 CFU/mL, followed by the addition of PMC and PMM at concentrations of 18 μg/mL, 36 μg/mL, or 72 μg/mL. The growth intensity was determined by measuring the optical density using a spectrophotometer over a period of 72 h. To determine the number of viable cells and their ability to ferment lactose, seeding on the Endo medium was performed. The biofilm-forming ability was determined on polystyrene plates using a staining and desorption method. The antibiotic susceptibility (ampicillin, levomycetin, meropenem, norfloxacin, and ceftriaxone) was determined using the disc-diffusion method for 24, 48, and 72 h of cultivation. Results. Both PMC and PMM exerted suppressive effects on the growth of E. coli B906: at a concentration of 72 μg/mL, the biomass increase was virtually absent. The number of viable cells on the medium with PMC decreased by 1—2 orders of magnitude at concentrations of 18 μg/mL and 36 μg/mL compared to the control and by 6 orders of magnitude at a concentration of 72 μg/mL. At this concentration, no growth was observed at 48 and 72 h. PMM exerted bacteriostatic effects: when seeded on the Endo medium, the number of viable cells decreased by 1—2 orders of magnitude at concentrations of 18 μg/mL and 36 μg/mL from 24 to 72 h, and by 3—4 orders of magnitude at a concentration of 72 μg/mL. At 48 h cultivation, PMC stimulated biofilm formation at concentrations of 18 μg/mL and 36 μg/mL, while inhibiting it at a concentration of 72 μg/mL. In contrast, PMM reduced the biofilm density at all concentrations. Both types of PM induced resistance to ampicillin, but the effect was stronger for PMM, which also led to resistance to norfloxacin. Conclusions. This study demonstrates that both PMC and PMM have a direct impact on lactose-positive E. coli strain B906, reflected in decreased growth intensity at moderate and high concentrations (36 μg/mL and 72 μg/mL) and increased aggressiveness through reduced enzymatic activity, enhanced biofilm formation, and the emergence of resistance to ampicillin, ceftriaxone, and norfloxacin.
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Mikrobiolohichnyi zhurnal
Mikrobiolohichnyi zhurnal Medicine-Microbiology (medical)
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