具有天然抗氧化剂潜力的木犀草(Sonneratia caseolaris)活性化合物的研究

M. Latief
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引用次数: 1

摘要

本研究旨在从海桑叶丙酮组分中分离出该化合物并测定其抗氧化活性。采用多级浸渍法提取。采用真空和重力柱色谱分离纯化,dpph法测定其抗氧化活性。通过紫外光谱、红外光谱和一、二维核磁共振光谱对化合物进行了结构分析。纯分离物外观为无臭的黄色晶体。根据光谱数据,该化合物为桑柱头-5,22-二烯-3-醇。ic50对提取物抗氧化剂和纯分离物的活性测定分别为166.2 ppm和134.4 ppm。
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The Characterization of Active Compound of Pedada Magrove Plants (Sonneratia caseolaris) Which HaveThePotential as Natural Antioxidants
The present research aimedto isolate the compound from the acetone fractions of pedada leaves (Sonneratia caseolaris) and test its antioxidant activity. The extraction wasperformed usinga multi-stage maceration method. The separation and purification were done using a vacuum and gravitational column chromatography whereasthe antioxidant activity was tested with theDPPH method. The structural elucidation of the compound was performed by spectroscopy data of UV, IR, alsoone- and two-dimensional NMR. The appearance of the pure isolate was odorless yellow crystal. Based on the spectroscopy data, the compound wasstigmasta-5,22-dien-3-ol. The activity testing of the extract antioxidant and pure isolate with IC50showed a value of 166.2 ppm dan 134.4 ppm, respectively.
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