波斯湾无壳海洋软体动物(Peronia peronii)甲醇提取物对黑色素瘤线粒体的毒性。

IF 1.6 4区 医学 Q3 OPHTHALMOLOGY Cutaneous and Ocular Toxicology Pub Date : 2023-03-01 DOI:10.1080/15569527.2022.2152041
Yalda Arast, Aida Jabbarzadeh, Farahnaz Tanbakosazan, Abdollah Arjmand, Amir Vazirizadeh, Jalal Pourahmad
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引用次数: 0

摘要

黑色素瘤是一种具有侵袭性和高致死率的癌症。预后差和治疗抵抗是黑色素瘤的特点。在黑色素瘤细胞中,严重依赖线粒体急性活性和活性氧(ROS)形成的凋亡信号被抑制。研究表明,从海洋草药和动物中分离出来的化合物,在先前的研究中已被证明对癌细胞具有细胞毒性。本研究旨在探讨波斯湾无壳海洋软体动物(Peronia peronii)甲醇提取物对黑色素瘤动物模型皮肤线粒体细胞凋亡的影响。目的:本研究从黑色素瘤动物模型皮肤中获得黑色素瘤线粒体,观察波斯湾无壳海洋软体动物(Peronia peronii)提取物是否对其具有细胞毒性作用。材料和方法:本研究采用差速离心法分离黑色素瘤细胞线粒体,用不同浓度(650、1300和2600µg/ml)的Peronia peronii甲醇提取物处理。然后进行MTT(3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑)活力测定、活性氧(ROS)测定、线粒体膜电位(MMP)下降测定、线粒体肿胀和细胞色素c释放测定。流式细胞术检测的%凋亡和坏死表型也进行了提取物处理的黑色素瘤细胞。结果:MTT实验结果显示,不同浓度的Peronia peronii提取物(P)显著增加了肿瘤组线粒体中ROS的生成、MMP的下降和细胞色素c的释放。与对照组相比,线粒体肿胀明显增加。此外,凋亡实验结果显示,添加Peronia peronii根提取物对黑色素瘤细胞凋亡增加,而对对照非肿瘤细胞无影响。讨论和结论:基于这些结果,Peronia peronii中潜在生物活性化合物的存在使这种波斯湾沿岸草药成为黑色素瘤癌症治疗领域进一步分子研究和临床研究的有力候选者。
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Toxicity of Persian Gulf shell-less marine mollusc (Peronia peronii) methanolic extract on melanoma tumor mitochondria.

Introduction: Melanoma is known as an aggressive and highly lethal cancer. The poor prognosis and resistance to treatment are characteristics of melanoma. In melanoma cells, apoptosis signaling which relies heavily on the acute activity of mitochondria and reactive oxygen species (ROS) formation is suppressed. Studies have shown that compounds isolated from marine herbs and animals, have been shown to have cytotoxic consequences on cancerous cells in prior research. This study was designed to evaluate the apoptotic effect of methanolic extract of Persian Gulf shell-less marine mollusc (Peronia peronii) on skin mitochondria isolated from animal model of melanoma.

Purpose: Melanoma mitochondria obtained from skin of melanoma animal model are studied in this research to see whether extracts from Persian Gulf shell-less marine mollusc (Peronia peronii), has a cytotoxic impact on them.

Material and method: In this study, the mitochondria were isolated from melanoma cells via differential centrifugation were treated with various concentrations (650, 1300 and 2600 µg/ml) of methanolic extract of Peronia peronii. Then MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) viability assay, Reactive oxygen species (ROS) determination, Mitochondrial Membrane Potential (MMP) decline assay, mitochondrial swelling and cytochrome c release determination were performed. Flow cytometry assay of % apoptotic vs necrotic phenotypes was also performed on extract treated melanoma cells.

Results: The results of MTT assay showed that different concentrations of Peronia peronii extract significantly (P < 0.05) decreased the SDH activity in cancerous skin mitochondria with the IC50(1300 μg/ml). The ROS results also showed that all concentrations of Peronia peronii extracts significantly increased ROS production, MMP decline and the release of cytochrome c in cancer groups mitochondria. The swelling of mitochondria was significantly increased compared to the control group. In addition, the results of apoptosis assay showed that addition of root extract of Peronia peronii on melanoma cells increased apoptosis, while it had no effect on control non tumour cells.

Discussion and conclusion: Based on these results, the presence of potentially bioactive compounds in Peronia peronii make this Persian Gulf coastal herb a strong candidate for further molecular studies and clinical research in the field of melanoma cancer therapy.

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来源期刊
CiteScore
3.30
自引率
6.20%
发文量
40
审稿时长
1 months
期刊介绍: Cutaneous and Ocular Toxicology is an international, peer-reviewed journal that covers all types of harm to cutaneous and ocular systems. Areas of particular interest include pharmaceutical and medical products; consumer, personal care, and household products; and issues in environmental and occupational exposures. In addition to original research papers, reviews and short communications are invited, as well as concise, relevant, and critical reviews of topics of contemporary significance.
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