A Comparison of efficiency of (AMP) and its derivative (AMPAA) against some pathogenic bacteria

Eugenol (4-allyl-2-methoxyphenol, AMP), is a well known a biologically active phenolic compo-nent and essential oil from Eugenia caryophyllata, which widely used of Eugenol as an antiseptic and analgesic in dental care, so it is active against oral bacteria associated with dental caries and periodontal disease as well as previous studies have shown the effect of Eugenol antifungal; anti-carcinogenic; anti-allergic; anti-mutagenic activity; antioxidant and insecticidal properties, there-fore it can be used in preparation of various food as a flavouring agent and cosmetic. This study aimed to synthesize derivative new medical material 2-(4-allyl-2-methoxyphenoxy) acetic acid (AMPAA) from eugenol (4-allyl-2-methoxyphenol) (AMP) and investigate the anti-microbial activities of both AMP and derivative component (AMPAA), The minimum inhibitory concentration (MIC); minimum bactericidal concentration (MBC) and sensitivity against six pathogenic bacterial isolates: Streptococcus. pyogens; Enterococcus. faecalis; Escherichia. coli ; Klebsiella pneumoniae; Bacillus. subtilis and Proteus. mirabilis with study compare the efficien-cy for both AMP and AMPAA on the same bacterial isolate obtained from Iraqi hospitals. In this present study synthesize new medical material (AMPAA) from AMP by reacting sodium eugenate with sodium chloroacetic acid and prepare soluble water from eugenol and its derivative AMPAAto scanning the antimicrobial efficacy against some pathogenic bacteria isolatesby two common methods; well diffusion and broth dilution methods. Results of the present study show all bacterial isolates were sensitive to both AMP and AMPAA in low concentration except K. pneumoniae, also 10.0 and 5.0 μg/mL of AMP and AMPAA as MBC for bacterial isolates except K. pneumoniae and P. mirabilis that’s meaning the new deriva-tive compound AMPAA has more efficacy on six bacterial isolate than eugenol (AMP). By using Well diffusion method all bacterial isolates were sensitive to both (AMP and AMPAA) in low concentration, but K. pneumoniae was killed in same concentration, so all isolates have been killed at concentrations between (10 - 50 μg/mL) of new derivative compound (AMPAA), at the same concentration of AMP were killed for K. pneumoniae and P. mirabilis) ,as well as all isolates have varying degrees of sensitivity towards both (AMP and AMPAA), whereas all iso-lates where more sensitive to AMPAA than AMP. In conclusion, all bacterial isolate were sensitive to both AMP and AMPAA in low concentration except K. pneumoniae, also 10.0 and 5.0 μg/mL of AMP and AMPAA respectively conceder as MBC for bacterial isolate except K. pneumoniae and P. mirabilis, that killed in these concentra-tion,so at the same concentration of AMP and AMPAA bacterial isolates were sensitivity by well diffusion method, whilst its killed by broth method, that’s consulate broth method was best than diffusion method