紫外-可见分光光度法测定辣木叶乙醇提取物中总黄酮含量

Wilda Ayu
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摘要

辣木(Moringa oleifera L.)是一种既可食用又可药用的植物。这种植物通常用作药物的部分是种子、叶子和树皮。采用紫外-可见分光光度法测定辣木叶乙醇提取物中总黄酮的含量。用96%乙醇浸渍法提取辣木叶。采用薄层色谱(TLC)法对辣木叶乙醇提取物进行植物化学筛选,紫外可见分光光度法测定其总黄酮含量。研究结果表明:辣木含有生物碱、类黄酮、单宁、游离类固醇/萜类和类固醇/萜类苷。每克辣木(Moringa oleifera, LamK)提取物总黄酮含量为32.261 gQE/g,提取率为5.11%。结论辣木叶中含有黄酮类化合物,提取物总黄酮含量为32.261 μgQE / g。
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DETERMINATION OF TOTAL FLAVONOID LEVELS OF ETHANOL EXTRACT MORINGA LEAVES (Moringa oleifera, LamK) BY SPECTROPHOTOMETRY UV-Vis
Moringa (Moringa oleifera L.) is one of the plants that can be used both as food and medicine. The parts of this plant which often used as medicine are the seeds, leaves, and barks. The purpose of this study was to determine the total flavonoid of the ethanol extract of Moringa leaves by UV-Vis spectrophotometry. Extraction method for Moringa leaves using maceration method with 96% ethanol solvent.Furthermore, phytochemical screening was carried out on the ethanolic extract of Moringa leaves using the Thin Layer Chromatography (TLC) method and determination of total flavonoid content by UV-Vis spectrophotometry. The results of the research are Moringa contains alkaloids, flavonoids, tannins, free steroids/terpenoids, and steroids/terpenoid glycosides. Each gram of Moringa (Moringa oleifera, LamK) contain a total flavonoid compound content of 32.261 gQE/g extract with a percentage yield of 5,11%. The conclusion that Moringa leaves contain flavonoids with total flavonoid content of 32.261 μgQE / g extract.
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