深耕条件下利用生物量合成水平筛选灵芝属真菌和赤霉素属真菌

K. Mustafin, N. Bisko, A. Kalieva, Z. Narmuratova, A. Zhakipbekova, Z. Saduyeva
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摘要

本文旨在寻找在不同成分的营养培养基上生长速度和生物量积累最活跃的药用灵芝和真菌菌株的收集培养物。对乌克兰国家科学院N.G. Kholodny植物研究所收集的蘑菇中不同地理来源的27株G. lucidum属和29株Trametes属(Fr.)进行了研究。研究了所研究菌株营养菌丝的径向生长速率及其在不同营养培养基上的培养和生长形态特征。在不同碳氮源培养基上,lucidum和T. versicolor真菌菌株的生物量积累水平存在一定差异。所有研究菌株都能在不同的碳氮源下生长。利用双因素经验的方差分析,可以详细说明营养源、菌株的生物学特性或它们的相互作用对生物量生物合成的影响。通过对菌丝菌落培养和形态特征、营养菌丝径向生长速率、生物量积累水平在6种液体营养培养基上的研究,发现在生物量积累方面产量最高的菌株是T. versicolor 353和G. lucidum 1621,选择这两种菌株进行进一步研究。
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Screening of fungi of the genus Ganoderma and Trametes by the level of biomass synthesis under conditions of deep cultivation
This article presents studies aimed at searching among the collection cultures of medicinal Ganoderma and Trametes fungal strains that are the most active in terms of growth rate and accumulation of biomass on nutrient media of various compositions. 27 strains of the genus G. lucidum of different geographical origins and 29 strains of the genus Trametes (Fr.) from the collection of mushrooms of the N.G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine were studied. The radial growth rate of vegetative mycelium of the studied strains and their cultural and morphological features of growth on different nutrient media were examined. Certain differences were established between G. lucidum and T. versicolor fungal strains in terms of the level of biomass accumulation on media with different carbon and nitrogen sources. All the studied strains are able to grow at different carbon and nitrogen sources. The use of variance analysis of two-factor experience made it possible to detail the influence of nutrient sources, biological characteristics of strains, or their interaction on biomass biosynthesis. Based on the study of the cultural and morphological features of mycelial colonies, the radial growth rate of vegetative mycelium, the level of biomass accumulation on six types of liquid nutrient media it was found that the most productive strains in terms of biomass accumulation were T. versicolor 353 and G. lucidum 1621, which were selected for further research.
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