Effects of Fenugreek (Trigonella foenumgraecum) Oil On Matrix Metalloprotease type 2 (MMP2) and Fibroblast growth factor type 2 (FGF2) expression during Wounds Healing Process in Diabetic Male Wister Rat

Background: Wound healing is a complicated process occurred to reform the integrity and homeostasis of wounded tissue, there is evidence that fenugreek induce and/or enhance the healing process in diabetic in male Wister rats. Aim: To investigate the effects of Fenugreek on levels of matrix metalloprotease type 2 (MMP2) and (FGF2) levels during wound healing process of diabetic male Wister rats via immunohistochemistry techniques. Methods: Thirty diabetic male Wister rats were divided into two main groups each group 15 rats, namely group D )diabetic rats without treatment ( and group FD )diabetic rats treated with 10% fenugreek oil applied locally on wound area(. Five rats were euthanized from each group at week two and week three of experimental period. MMP2 and FGF2 expression levels were measured using immunohistochemistry technique. Results: Fenugreek oil showed the ability to decrease the expression of MMP2 in 10% fenugreek oil treated diabetic rats during week two and week three of wound healing process compared with diabetic rats without treatment. However, Fenugreek oil stimulated high expression of FGF2 in 10% fenugreek oil treated diabetic rats during experimental period compared with diabetic rats without treatment. Conclusion: The results of the present study revealed that fenugreek oil treatment has the ability to reduce the expression of MMP2 and elevate FGF2 expression in 10% fenugreek oil treated diabetic rats compared with non-treated diabetic rats.