由Rhizopus oryzae fnycc 6078生成的fibrino对Tempe酶

Alicia Sada, Noor Erma Sugianto, A. Poernomo
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引用次数: 1

摘要

对米根霉FNCC 6078进行了固体发酵产纤溶酶的研究。以大豆为原料,在豆豉中发酵生产纤溶酶。本研究的主要目的是揭示发酵的最佳条件。条件参数为接种量、孵育时间和温度。以最大纤溶活性确定最佳条件。方法采用分光光度法测定纤维蛋白溶解活性。结果产纤溶酶的最佳条件为接种量为1.5 mL的米根霉混悬液,温度为25%,孵育42 h,温度为35℃。
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Produksi Enzim Fibrinolitik Tempe oleh Rhizopus oryzae FNCC 6078
ackground Rhizopus oryzae FNCC 6078 had been evaluated producing fibrinolytic enzyme under solid state fermentation. Soybean had been used to produce fibrinolytic enzyme through fermentation in tempeh. The main purpose of this study was to reveal optimum condition for fermentation. The parameters of the condition were inoculum volume, incubation period and temperature. Optimum condition was defined by maximum fibrinolytic activity. Methode Fibrinolytic activity was measured using spectrophotometry at 274 nm. Result optimum condition for producing fibrinolytic enzyme was 1,5 mL volume of inoculum of Rhizopus oryzae suspension in 25%T, 42 hours for incubation period and 35oC temperature incubation.
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