亲脂铋纳米颗粒与十六烷基吡啶氯化抑制肺癌生长的累积抗肿瘤作用。

IF 3.1 4区 医学 Q2 BIOPHYSICS Journal of Applied Biomaterials & Functional Materials Pub Date : 2023-01-01 DOI:10.1177/22808000231161177
Claudia María García-Cuellar, Rene Hernández-Delgadillo, Jesús Alejandro Torres-Betancourt, Juan Manuel Solis-Soto, Irene Meester, Yesennia Sánchez-Pérez, Nayely Pineda-Aguilar, Sergio Eduardo Nakagoshi-Cepeda, Rosa Isela Sánchez-Nájera, María Argelia Akemi Nakagoshi-Cepeda, Shankararaman Chellam, Claudio Cabral-Romero
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引用次数: 0

摘要

目的:探讨亲脂铋纳米粒子(BisBAL NP)与氯化十六烷基吡啶(CPC)对人肺肿瘤细胞的联合抑瘤作用。材料和方法:将人肺肿瘤细胞A549分别暴露于1-100µM BisBAL NP或CPC中,或以1:1的比例组合。采用PrestoBlue法、LIVE/DEAD法和荧光显微镜检测细胞活力。免疫荧光分析细胞微管的完整性和形态。结果:暴露于1µM溶液24 h, BisBAL NP对A549细胞的生长抑制率为21.5%,CPC为70.5%,联合作用为92.4% (p p)。结论:BisBAL NP和CPC对A549人肿瘤细胞的生长抑制作用自1µM开始累积。BisBAL NP/CPC组合可能是治疗人类肺癌的一种创新且具有成本效益的替代方案。
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Cumulative antitumor effect of bismuth lipophilic nanoparticles and cetylpyridinium chloride in inhibiting the growth of lung cancer.

Objective: To determine the combined antitumor effect of bismuth lipophilic nanoparticles (BisBAL NP) and cetylpyridinium chloride (CPC) on human lung tumor cells.

Material and methods: The human lung tumor cells A549 were exposed to 1-100 µM BisBAL NP or CPC, either separately or in a 1:1 combination. Cell viability was measured with the PrestoBlue assay, the LIVE/DEAD assay, and fluorescence microscopy. The integrity and morphology of cellular microtubules were analyzed by immunofluorescence.

Results: A 24-h exposure to 1 µM solutions reduced A549 growth with 21.5% for BisBAL NP, 70.5% for CPC, and 92.4% for the combination (p < 0.0001), while a 50 µM BisBAL NP/CPC mixture inhibited cell growth with 99% (p < 0.0001). BisBAL NP-curcumin conjugates were internalized within 30 min of exposure and could be traced within the nucleus of tumor cells within 2 h. BisBAL NP, but not CPC, interfered with microtubule organization, thus interrupting cell replication, similar to the action mechanism of docetaxel.

Conclusion: The growth inhibition of A549 human tumor cells by BisBAL NP and CPC was cumulative as of 1 µM. The BisBAL NP/CPC combination may constitute an innovative and cost-effective alternative for treating human lung cancer.

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来源期刊
Journal of Applied Biomaterials & Functional Materials
Journal of Applied Biomaterials & Functional Materials BIOPHYSICS-ENGINEERING, BIOMEDICAL
CiteScore
4.40
自引率
4.00%
发文量
36
审稿时长
>12 weeks
期刊介绍: The Journal of Applied Biomaterials & Functional Materials (JABFM) is an open access, peer-reviewed, international journal considering the publication of original contributions, reviews and editorials dealing with clinical and laboratory investigations in the fast growing field of biomaterial sciences and functional materials. The areas covered by the journal will include: • Biomaterials / Materials for biomedical applications • Functional materials • Hybrid and composite materials • Soft materials • Hydrogels • Nanomaterials • Gene delivery • Nonodevices • Metamaterials • Active coatings • Surface functionalization • Tissue engineering • Cell delivery/cell encapsulation systems • 3D printing materials • Material characterization • Biomechanics
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