Determination of daunorubicin in human urine by capillary zone electrophoresis with amperometric detection

A simple, reliable and reproducible method, based on capillary zone electrophoresis with amperometric detection, for the determination of daunorubicin in human urine is described in this paper. A carbon disk electrode used as working electrode. The optimal conditions of separation and detection were pH 7.8 phosphate buffer (0.15 mol/l), 8 kV for the separation voltage and 0.95 V (versus Ag/AgCl, 3 mol/l KCl) for the detection potential. The linear range was from 2×10⁻⁶ to 2×10⁻⁴ mol/l with the regression coefficient of 0.9995, and the detection limit was 8×10⁻⁷ mol/l. The method built in this paper was directly applied to the determination of daunorubicin in the imitation human urine without any other sample pre-treatment except filtration, and the assay result was satisfactory.