更正“在小鼠中产生OMgp等位基因系列”

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2023-03-20 DOI:10.1002/dvg.23513
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引用次数: 0

摘要

Lee, J.K, Case, L.C, Chan, A.F, Zhu, Y., Tessier-Lavigne, M.和Zheng, B.(2009),小鼠OMgp等位基因序列的生成。创世纪,47:751-756。https://doi.org/10.1002/dvg.20557在最初发表的文章中,在图3a和图4b所示的两个Western blots上α-Tubulin对照存在错误。同时运行两组凝胶,每组一份(共4个凝胶),并依次用OMgp抗体和对照α-微管蛋白抗体进行印迹。根据突变条件,OMgp信号在不同的样品中给出了不同的模式,而控制α-微管蛋白的信号给出了相似的模式,这导致了α-微管蛋白控制的特定通道的数字混乱和错误。对感兴趣的蛋白质OMgp没有错误。作者追踪了α-微管蛋白控制的正确通道,图3a和图4a的正确Western blot图像,以及图3b和图4b的更新定量,如下图所示。除以下内容外,两个图的图例保持相同:所示为3 - 4组(而不是3组)独立生物样本中的一组的代表性数据。这些错误对论文的科学结论没有影响。我们为这个错误道歉。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Correction to “Generation of an OMgp allelic series in mice”

Lee, J.K., Case, L.C., Chan, A.F., Zhu, Y., Tessier-Lavigne, M. and Zheng, B. (2009), Generation of an OMgp allelic series in mice. Genesis, 47: 751756. https://doi.org/10.1002/dvg.20557

In the originally published article, there are errors in the α-Tubulin controls on two Western blots presented in Figure 3a and Figure 4b. Two sets of gels, each in duplicate (a total of four gels), were run at the same time, and all were blotted sequentially with antibodies to OMgp and control α-Tubulin. While OMgp signals gave distinct patterns across different samples depending on the mutant conditions, control α-Tubulin signals gave similar patterns, which led to confusion and therefore errors in the figures in the specific lanes used for α-Tubulin controls. There were no errors for OMgp, which was the protein of interest. The authors have tracked the correct lanes for α-Tubulin controls, and the correct Western blot images for Figure 3a and Figure 4a, along with updated quantifications in Figure 3b and Figure 4b, is shown below. The figure legends remain the same for both figures except for the following: Representative data from one of 3–4 (instead of 3) independent sets of biological samples are shown. These errors had no impact on the scientific conclusions of the paper.

We apologize for this error.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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