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{"title":"多能干细胞多巴胺能神经元与星形胶质细胞的制备与共培养","authors":"Aurelie de Rus Jacquet","doi":"10.1002/cpcb.98","DOIUrl":null,"url":null,"abstract":"<p>Induced pluripotent stem cell (iPSC)-based models are powerful tools to study neurodegenerative diseases such as Parkinson's disease. The differentiation of patient-derived neurons and astrocytes allows investigation of the molecular mechanisms responsible for disease onset and development. In particular, these two cell types can be mono- or co-cultured to study the influence of cell-autonomous and non-cell-autonomous contributors to neurodegenerative diseases. We developed a streamlined procedure to produce high-quality/high-purity cultures of dopaminergic neurons and astrocytes that originate from the same population of midbrain floor-plate progenitors. This unit describes differentiation, quality control, culture parameters, and troubleshooting tips to ensure the highest quality and reproducibility of research results. © 2019 The Authors.</p><p><b>Basic Protocol 1</b>: Differentiation of iPSCs into midbrain-patterned neural progenitor cells</p><p><b>Support Protocol</b>: Quality control of neural progenitor cells</p><p><b>Basic Protocol 2</b>: Differentiation of neural progenitor cells into astrocytes</p><p><b>Basic Protocol 3</b>: Differentiation of neural progenitor cells into dopaminergic neurons</p><p><b>Basic Protocol 4</b>: Co-culture of iPSC-derived neurons and astrocytes</p>","PeriodicalId":40051,"journal":{"name":"Current Protocols in Cell Biology","volume":"85 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpcb.98","citationCount":"14","resultStr":"{\"title\":\"Preparation and Co-Culture of iPSC-Derived Dopaminergic Neurons and Astrocytes\",\"authors\":\"Aurelie de Rus Jacquet\",\"doi\":\"10.1002/cpcb.98\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Induced pluripotent stem cell (iPSC)-based models are powerful tools to study neurodegenerative diseases such as Parkinson's disease. The differentiation of patient-derived neurons and astrocytes allows investigation of the molecular mechanisms responsible for disease onset and development. In particular, these two cell types can be mono- or co-cultured to study the influence of cell-autonomous and non-cell-autonomous contributors to neurodegenerative diseases. We developed a streamlined procedure to produce high-quality/high-purity cultures of dopaminergic neurons and astrocytes that originate from the same population of midbrain floor-plate progenitors. This unit describes differentiation, quality control, culture parameters, and troubleshooting tips to ensure the highest quality and reproducibility of research results. © 2019 The Authors.</p><p><b>Basic Protocol 1</b>: Differentiation of iPSCs into midbrain-patterned neural progenitor cells</p><p><b>Support Protocol</b>: Quality control of neural progenitor cells</p><p><b>Basic Protocol 2</b>: Differentiation of neural progenitor cells into astrocytes</p><p><b>Basic Protocol 3</b>: Differentiation of neural progenitor cells into dopaminergic neurons</p><p><b>Basic Protocol 4</b>: Co-culture of iPSC-derived neurons and astrocytes</p>\",\"PeriodicalId\":40051,\"journal\":{\"name\":\"Current Protocols in Cell Biology\",\"volume\":\"85 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-10-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/cpcb.98\",\"citationCount\":\"14\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Protocols in Cell Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/cpcb.98\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Cell Biology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpcb.98","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 14
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Preparation and Co-Culture of iPSC-Derived Dopaminergic Neurons and Astrocytes
Induced pluripotent stem cell (iPSC)-based models are powerful tools to study neurodegenerative diseases such as Parkinson's disease. The differentiation of patient-derived neurons and astrocytes allows investigation of the molecular mechanisms responsible for disease onset and development. In particular, these two cell types can be mono- or co-cultured to study the influence of cell-autonomous and non-cell-autonomous contributors to neurodegenerative diseases. We developed a streamlined procedure to produce high-quality/high-purity cultures of dopaminergic neurons and astrocytes that originate from the same population of midbrain floor-plate progenitors. This unit describes differentiation, quality control, culture parameters, and troubleshooting tips to ensure the highest quality and reproducibility of research results. © 2019 The Authors.
Basic Protocol 1 : Differentiation of iPSCs into midbrain-patterned neural progenitor cells
Support Protocol : Quality control of neural progenitor cells
Basic Protocol 2 : Differentiation of neural progenitor cells into astrocytes
Basic Protocol 3 : Differentiation of neural progenitor cells into dopaminergic neurons
Basic Protocol 4 : Co-culture of iPSC-derived neurons and astrocytes
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