阿喀琉叶乙酸乙酯部分诱导人宫颈癌(HeLa)细胞凋亡和细胞周期阻滞

IF 3.5 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Annals of Agricultural Science Pub Date : 2020-06-01 DOI:10.1016/j.aoas.2020.03.003
Doha H. Abou Baker
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引用次数: 22

摘要

采用高效液相色谱法(HPLC)对不同溶剂(石油醚(pet-ether)、乙酸乙酯(EtOAc)、甲醇(MeOH)和水)提取的千叶阿基那(Achillea millefolium L)馏分中20种活性成分进行了综合定量分析。此外,采用MTT法(3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑)测定了千叶提取物对k562、HeLa、MCF7、A431和A549细胞株的总黄酮(TF)、总酚类物质(TP)、抗氧化剂(DPPH、ABTS)和细胞毒活性,并以阿霉素为对照。结果表明,TP值为3.8 ~ 91.85 mg没食子酸当量(GAE)/g, TF值为9.68 ~ 79.50 mg儿茶素当量(CE)/g。除EtOAc部分外,阿霉素对Hela细胞的IC50值最低,其次是水部分,其对MCF-7细胞的抗癌潜力最高。pet-醚组分对K562细胞的IC50值最低。在HeLa细胞中研究了EtOAc组分的细胞周期谱和凋亡分析。EtOAc组分引起HeLa癌细胞g1前期凋亡和G2/M细胞生长阻滞。综上所述,本研究表明,千叶草是一种很有前途的天然抗氧化剂和抗癌药物来源,在未来开发新的功能性和治疗性化合物中具有重要意义。
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Achillea millefolium L. ethyl acetate fraction induces apoptosis and cell cycle arrest in human cervical cancer (HeLa) cells

This study aimed to develop a comprehensive quantification of 20 bioactive compounds in Achillea millefolium L (A. millefolium) fractions obtained by different solvents (petroleum ether (pet-ether), ethyl acetate (EtOAc), methanol (MeOH) and water) using HPLC quantitative analysis. Additionally, total flavonoids (TF), total phenolics (TP), antioxidants (DPPH, ABTS) and cytotoxic activities of A. millefolium fractions on k562, HeLa, MCF7, A431 and A549 Cell Lines were evaluated using MTT assay [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and compared to doxorubicin as a standard. According to the obtained results, TP ranged from 3.8 to 91.85 mg gallic acid equivalent (GAE)/g fraction, while TF ranged from 9.68 to 79.50 mg catechin equivalent (CE)/g generally. The lowest IC50 values were recorded for doxorubicin compared by all fractions except for EtOAc fraction against Hela cells, followed by water fraction, which showed the highest anticancer potential against MCF-7 cells. In contrast, the pet-ether fraction showed the lowest IC50 value against K562 cells. The cell cycle profile and apoptosis analysis of the EtOAc fraction was studied in HeLa cells. EtOAc fraction caused preG1 apoptosis and cell growth arrest in G2/M in HeLa cancer cells. Overall, this study heightens the importance of A. millefolium, which may be a promising and valuable source of natural antioxidant and anticancer agents to be used in the development of new functional and therapeutic compounds in the future.

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来源期刊
Annals of Agricultural Science
Annals of Agricultural Science AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
12.60
自引率
0.00%
发文量
18
审稿时长
33 days
期刊介绍: Annals of Agricultural Sciences (AOAS) is the official journal of Faculty of Agriculture, Ain Shams University. AOAS is an open access peer-reviewed journal publishing original research articles and review articles on experimental and modelling research at laboratory, field, farm, landscape, and industrial levels. AOAS aims to maximize the quality of the agricultural sector across the globe with emphasis on the Arabian countries by focusing on publishing the high-quality applicable researches, in addition to the new methods and frontiers leading to maximizing the quality and quantity of both plant and animal yield and final products.
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