{"title":"利用platarthrospira(螺旋藻)残基水解物补充l -酪氨酸优化链霉菌NRRL B-1701产黑素","authors":"Oranit Kraseasintra, Sritip Sensupa, Kanjana Mahanil, Sada Yoosathaporn, Jeeraporn Pekkoh, Sirasit Srinuanpan, Wasu Pathom-Aree, Chayakorn Pumas","doi":"10.3390/biotech12010024","DOIUrl":null,"url":null,"abstract":"<p><p>Melanin is a functional pigment that is used in various products. It can be produced by <i>Streptomyces antibioticus</i> NRRL B-1701 when supplemented with L-tyrosine. <i>Arthrospira (Spirulina) platensis</i> is a cyanobacterium with high protein content, including the protein phycocyanin (PC). During PC's extraction, biomass residues are generated, and these residues still contain various amino acids, especially L-tyrosine, which can be used as a low-cost supplement for melanin production. Thus, this study employed a hydrolysate of <i>A. platensis</i> biomass residue for L-tyrosine substitution. The effects of two drying methods, namely, lyophilization and dying via a hot air oven, on the proximate composition and content of L-tyrosine in the biomass residue were evaluated. The highest L-tyrosine (0.268 g L-tyrosine/100 g dried biomass) concentration was obtained from a hot-air-oven-dried biomass residue hydrolysate (HAO-DBRH). The HAO-DBRH was then used as a low-cost L-tyrosine supplement for maximizing melanin production, which was optimized by the response surface methodology (RSM) through central composite design (CCD). Using the RSM-CCD, the maximum level of melanin production achieved was 0.24 g/L, which is approximately four times higher than it was before optimization. This result suggests that <i>A. platensis</i> residue hydrolysate could be an economically feasible and low-cost alternative source of L-tyrosine for the production of melanin.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10046677/pdf/","citationCount":"0","resultStr":"{\"title\":\"Optimization of Melanin Production by <i>Streptomyces antibioticus</i> NRRL B-1701 Using <i>Arthrospira (Spirulina) platensis</i> Residues Hydrolysates as Low-Cost L-tyrosine Supplement.\",\"authors\":\"Oranit Kraseasintra, Sritip Sensupa, Kanjana Mahanil, Sada Yoosathaporn, Jeeraporn Pekkoh, Sirasit Srinuanpan, Wasu Pathom-Aree, Chayakorn Pumas\",\"doi\":\"10.3390/biotech12010024\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Melanin is a functional pigment that is used in various products. It can be produced by <i>Streptomyces antibioticus</i> NRRL B-1701 when supplemented with L-tyrosine. <i>Arthrospira (Spirulina) platensis</i> is a cyanobacterium with high protein content, including the protein phycocyanin (PC). During PC's extraction, biomass residues are generated, and these residues still contain various amino acids, especially L-tyrosine, which can be used as a low-cost supplement for melanin production. Thus, this study employed a hydrolysate of <i>A. platensis</i> biomass residue for L-tyrosine substitution. The effects of two drying methods, namely, lyophilization and dying via a hot air oven, on the proximate composition and content of L-tyrosine in the biomass residue were evaluated. The highest L-tyrosine (0.268 g L-tyrosine/100 g dried biomass) concentration was obtained from a hot-air-oven-dried biomass residue hydrolysate (HAO-DBRH). The HAO-DBRH was then used as a low-cost L-tyrosine supplement for maximizing melanin production, which was optimized by the response surface methodology (RSM) through central composite design (CCD). Using the RSM-CCD, the maximum level of melanin production achieved was 0.24 g/L, which is approximately four times higher than it was before optimization. This result suggests that <i>A. platensis</i> residue hydrolysate could be an economically feasible and low-cost alternative source of L-tyrosine for the production of melanin.</p>\",\"PeriodicalId\":34490,\"journal\":{\"name\":\"BioTech\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2023-03-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10046677/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BioTech\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/biotech12010024\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BioTech","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/biotech12010024","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
黑色素是一种用于各种产品的功能性色素。在添加l -酪氨酸的条件下,链霉菌NRRL B-1701可产生该菌。Arthrospira Arthrospira platensis是一种蛋白质含量高的蓝藻菌,其中包括蛋白质藻蓝蛋白(PC)。PC在提取过程中会产生生物质残渣,这些残渣中仍然含有多种氨基酸,尤其是l -酪氨酸,可以作为黑色素生产的低成本补充。因此,本研究采用platensis生物质渣的水解产物替代l -酪氨酸。研究了冻干和热风炉干燥两种干燥方法对生物质残渣中l -酪氨酸近似组成和含量的影响。热风炉干燥生物质残渣水解液(HAO-DBRH)的l -酪氨酸浓度最高(0.268 g l -酪氨酸/100 g干燥生物质)。然后将HAO-DBRH作为低成本的l -酪氨酸补充剂,通过响应面法(RSM)通过中心复合设计(CCD)对其进行优化,以最大化黑色素的产生。使用RSM-CCD,获得的最大黑色素产量为0.24 g/L,比优化前提高了约4倍。这一结果表明,platensis残渣水解物可能是一种经济可行且低成本的l -酪氨酸替代来源,用于黑色素的生产。
Optimization of Melanin Production by Streptomyces antibioticus NRRL B-1701 Using Arthrospira (Spirulina) platensis Residues Hydrolysates as Low-Cost L-tyrosine Supplement.
Melanin is a functional pigment that is used in various products. It can be produced by Streptomyces antibioticus NRRL B-1701 when supplemented with L-tyrosine. Arthrospira (Spirulina) platensis is a cyanobacterium with high protein content, including the protein phycocyanin (PC). During PC's extraction, biomass residues are generated, and these residues still contain various amino acids, especially L-tyrosine, which can be used as a low-cost supplement for melanin production. Thus, this study employed a hydrolysate of A. platensis biomass residue for L-tyrosine substitution. The effects of two drying methods, namely, lyophilization and dying via a hot air oven, on the proximate composition and content of L-tyrosine in the biomass residue were evaluated. The highest L-tyrosine (0.268 g L-tyrosine/100 g dried biomass) concentration was obtained from a hot-air-oven-dried biomass residue hydrolysate (HAO-DBRH). The HAO-DBRH was then used as a low-cost L-tyrosine supplement for maximizing melanin production, which was optimized by the response surface methodology (RSM) through central composite design (CCD). Using the RSM-CCD, the maximum level of melanin production achieved was 0.24 g/L, which is approximately four times higher than it was before optimization. This result suggests that A. platensis residue hydrolysate could be an economically feasible and low-cost alternative source of L-tyrosine for the production of melanin.