利用Phos-Tag凝胶分析蛋白质磷酸化

Q1 Biochemistry, Genetics and Molecular Biology Current Protocols in Protein Science Pub Date : 2018-07-25 DOI:10.1002/cpps.64

Zoltan Nagy, Shane Comer, Albert Smolenski

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Analysis of Protein Phosphorylation Using Phos-Tag Gels

Phos-tag gels are recent tools to dissect protein phosphorylation that operate by inducing a shift in the electrophoretic mobility of phosphorylated proteins compared to their nonphosphorylated counterparts. This article describes the preparation and electrophoresis of Zn²⁺-Phos-tag gels along with electrotransfer of the separated phospho- and nonphosphoproteins onto a PVDF membrane using either wet-tank or semidry transfer. We also discuss the theory behind the technology with critical parameters to keep in mind for its successful application. © 2018 by John Wiley & Sons, Inc.

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Current Protocols in Protein Science Biochemistry, Genetics and Molecular Biology-Biochemistry

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期刊介绍： With the mapping of the human genome, more and more researchers are exploring protein structures and functions in living organisms. Current Protocols in Protein Science provides protein scientists, biochemists, molecular biologists, geneticists, and others with the first comprehensive suite of protocols for this growing field.

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