Burak Alaylar, Bünyamin Aygün, Kadir Turhan, Mehmet Karadayı, Esra Cinan, Zuhal Turgut, Gökçe Karadayı, Mohammed Ibrahim Abu Al-Sayyed, Medine Güllüce, Abdulhalik Karabulut
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引用次数: 1
摘要
目的:分析了五种不同类型的合成azadispiro衍生物的辐射吸收能力，并确定了它们作为抗辐射药物开发物质的潜力。材料与方法:利用Monte Carlo simulation Geometry and Tracking (GEANT4)程序计算快中子衰减参数，如有效平均自由程、半值层(HVL)、去除截面、中子透射数等。利用WinXCom软件对有效原子序数(Zeff)、平均自由程(MFP)、质量衰减系数(MAC)、半值层(HVL)等γ辐射吸收参数进行理论测定。此外，利用GP拟合参数计算暴露累积因子(EBF)。利用能量为4.5 MeV、活度为74 GBq的241Am-Be快中子源和便携式BF3中子探测器，实验计算了中子吸收剂量率。采用Ames/沙门氏菌检测系统检测阿扎皮罗衍生物的遗传毒性。结果与结论:用石蜡和高密度聚乙烯对实验和理论结果进行了验证。结果表明，Azadispiro衍生物具有接近石蜡和高密度聚乙烯的中子辐射吸收能力。研究了azadispiro衍生物对γ辐射的吸收特性，发现这些材料可以吸收γ辐射。采用Ames/沙门氏菌法检测衍生物是否具有遗传毒性作用概率。结果表明，这些衍生物在试验剂量(高达5 mM)下具有遗传毒性和安全性。因此，人们已经认识到，这些azadispiro衍生物可以作为活性和遗传毒性的安全成分，用于生产抗中子和伽马射线的保护药物。
Investigation of radiation protective features of azadispiro derivatives and their genotoxic potential with Ames/Salmonella test system.
Purpose: Five different types of synthesized azadispiro derivatives have been analyzed for radiation absorption capacity and determined their potential to be exploited as substances for a drug to be developed against radiation has been investigated.
Material and methods: Fast neutron attenuation parameters like the effective mean free path, half-value layer (HVL), removal cross-sections, and neutron transmission number were found with the Monte Carlo simulation Geometry And Tracking (GEANT4) code. Gamma radiation absorption parameters, such as effective atom number (Zeff), mean free path (MFP), mass attenuation coefficient (MAC), and half-value layer (HVL) were theoretically determined with WinXCom software. Besides, the exposure build-up factor (EBF) was calculated by using GP fitting parameters. Neutron absorption dose rate was experimentally calculated with 241Am-Be fast neutron source which has 4.5 MeV of energy, 74 GBq activity, and portative BF3 neutron detector. Ames/Salmonella test systems were used for the genotoxic potentials of the azadispiro derivatives.
Results and conclusions: Experimental and theoretical results were checked with paraffin and High-Density Polyethylene. The results showed that Azadispiro derivatives have neutron radiation absorption capability close to paraffin and High-Density Polyethylene. The gamma radiation absorption properties for azadispiro derivatives have been investigated, and it has been observed that these materials can absorb gamma radiation. Ames/Salmonella assay was used to examine whether the derivatives had a genotoxic effect probability or not. The results showed that these derivatives were genotoxic and safe at test doses (up to 5 mM). Consequently, it has been understood that these azadispiro derivatives can be used as active and genotoxic safety ingredients in the production of a protective drug against both neutrons and gamma rays.
期刊介绍：
The International Journal of Radiation Biology publishes original papers, reviews, current topic articles, technical notes/reports, and meeting reports on the effects of ionizing, UV and visible radiation, accelerated particles, electromagnetic fields, ultrasound, heat and related modalities. The focus is on the biological effects of such radiations: from radiation chemistry to the spectrum of responses of living organisms and underlying mechanisms, including genetic abnormalities, repair phenomena, cell death, dose modifying agents and tissue responses. Application of basic studies to medical uses of radiation extends the coverage to practical problems such as physical and chemical adjuvants which improve the effectiveness of radiation in cancer therapy. Assessment of the hazards of low doses of radiation is also considered.
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