分析荧光法中的双发射及其λ-比率检测。第1章报告信号产生的基本机制。

IF 2.4 3区 化学 Q3 CHEMISTRY, ANALYTICAL Methods and Applications in Fluorescence Pub Date : 2023-04-21 DOI:10.1088/2050-6120/acc714
Alexander Demchenko
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引用次数: 1

摘要

波长比技术在荧光探测和传感中越来越受欢迎,因为它为输出信号提供了内部参考,并消除了仪器伪影,从而提高了检测的灵敏度和可靠性。最近的发展表明,随着广泛的新型分子和纳米级荧光团(发光团)的应用,这种方法可以实现更多的目标,探索光物理和光化学效应的全部力量,并使用更广泛的分析格式。检测的简单性和输出数据的潜在丰富内容允许在不同的简化、小型化和多路复用设备中实现这些技术。后一个问题将在本系列的第二章中讨论。
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Dual emission and itsλ-ratiometric detection in analytical fluorimetry. Pt. I. Basic mechanisms of generating the reporter signal.

The wavelength-ratiometric techniques gain increasing popularity in fluorescence probing and sensing for providing inner reference to output signal and removing instrumental artefacts, in this way increasing the sensitivity and reliability of assays. Recent developments demonstrate that such approach can allow achieving much more, with the application of broad range of novel molecular and nanoscale fluorophores (luminophores), exploring the whole power of photophysical and photochemical effects and using extended range of assay formats. Simplicity of detection and potentially rich content of output data allows realizing these techniques in different simplified, miniaturized and multiplexing devices. The latter issues are discussed in Pt. II of these series.

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来源期刊
Methods and Applications in Fluorescence
Methods and Applications in Fluorescence CHEMISTRY, ANALYTICALCHEMISTRY, PHYSICAL&n-CHEMISTRY, PHYSICAL
CiteScore
6.20
自引率
3.10%
发文量
60
期刊介绍: Methods and Applications in Fluorescence focuses on new developments in fluorescence spectroscopy, imaging, microscopy, fluorescent probes, labels and (nano)materials. It will feature both methods and advanced (bio)applications and accepts original research articles, reviews and technical notes.
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