{"title":"插入多态性揭示的黄花蒿遗传多样性和种群结构的分子见解。","authors":"Hossein Abbasi Holasou, Negar Valizadeh, Seyyed Abolghasem Mohammadi","doi":"10.1007/s40415-022-00860-x","DOIUrl":null,"url":null,"abstract":"<p><p>The knowledge about the level of genetic diversity and population structure in natural populations of <i>Artemisia annua</i> L. is a primary step in breeding programs for development of new cultivars with higher artemisinin level and better quality of secondary metabolites composition. We used PCR-based \"retrotransposon-microsatellite amplified polymorphisms\" (REMAPs) to study insertional polymorphism in <i>A. annua</i> genome to assess genetic variability and population structure in a collection of 118 accessions collected from north and northwest of Iran. Twenty-five primer combinations of 10 retrotransposon and seven ISSR primers amplified a total of 693 clear and unambiguous fragments in the studied accessions. The average number of bands, polymorphic bands, polymorphism, effective number of alleles, Shannon's information index and expected heterozygosity were 27.72, 24.76, 88.14%, 1.47, 0.42 and 0.28, respectively. The analysis of molecular variance revealed high genetic variation present within sampled geographical regions. Distance-based cluster analysis assigned the studied accessions into four clusters according to their geographical origin, which were also confirmed by principal coordinate analysis. In model-based Bayesian clustering, the maximum value of Δ<i>k</i> was obtained when the collection of 118 assayed <i>A. annua</i> accessions assigned into two subgroups (<i>K</i> = 2). The results showed the high genetic variation in the collection of Iranian sweet wormwood which revealed by REMAP markers indicating the reliability and efficiency of this marker system for analysis of genetic diversity and population structure of <i>A. annua</i>.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s40415-022-00860-x.</p>","PeriodicalId":9140,"journal":{"name":"Brazilian Journal of Botany","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9807429/pdf/","citationCount":"0","resultStr":"{\"title\":\"Molecular insights into the genetic diversity and population structure of <i>Artemisia annua</i> L. as revealed by insertional polymorphisms.\",\"authors\":\"Hossein Abbasi Holasou, Negar Valizadeh, Seyyed Abolghasem Mohammadi\",\"doi\":\"10.1007/s40415-022-00860-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The knowledge about the level of genetic diversity and population structure in natural populations of <i>Artemisia annua</i> L. is a primary step in breeding programs for development of new cultivars with higher artemisinin level and better quality of secondary metabolites composition. We used PCR-based \\\"retrotransposon-microsatellite amplified polymorphisms\\\" (REMAPs) to study insertional polymorphism in <i>A. annua</i> genome to assess genetic variability and population structure in a collection of 118 accessions collected from north and northwest of Iran. Twenty-five primer combinations of 10 retrotransposon and seven ISSR primers amplified a total of 693 clear and unambiguous fragments in the studied accessions. The average number of bands, polymorphic bands, polymorphism, effective number of alleles, Shannon's information index and expected heterozygosity were 27.72, 24.76, 88.14%, 1.47, 0.42 and 0.28, respectively. The analysis of molecular variance revealed high genetic variation present within sampled geographical regions. Distance-based cluster analysis assigned the studied accessions into four clusters according to their geographical origin, which were also confirmed by principal coordinate analysis. In model-based Bayesian clustering, the maximum value of Δ<i>k</i> was obtained when the collection of 118 assayed <i>A. annua</i> accessions assigned into two subgroups (<i>K</i> = 2). The results showed the high genetic variation in the collection of Iranian sweet wormwood which revealed by REMAP markers indicating the reliability and efficiency of this marker system for analysis of genetic diversity and population structure of <i>A. annua</i>.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s40415-022-00860-x.</p>\",\"PeriodicalId\":9140,\"journal\":{\"name\":\"Brazilian Journal of Botany\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9807429/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Brazilian Journal of Botany\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s40415-022-00860-x\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/1/3 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brazilian Journal of Botany","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s40415-022-00860-x","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/1/3 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Molecular insights into the genetic diversity and population structure of Artemisia annua L. as revealed by insertional polymorphisms.
The knowledge about the level of genetic diversity and population structure in natural populations of Artemisia annua L. is a primary step in breeding programs for development of new cultivars with higher artemisinin level and better quality of secondary metabolites composition. We used PCR-based "retrotransposon-microsatellite amplified polymorphisms" (REMAPs) to study insertional polymorphism in A. annua genome to assess genetic variability and population structure in a collection of 118 accessions collected from north and northwest of Iran. Twenty-five primer combinations of 10 retrotransposon and seven ISSR primers amplified a total of 693 clear and unambiguous fragments in the studied accessions. The average number of bands, polymorphic bands, polymorphism, effective number of alleles, Shannon's information index and expected heterozygosity were 27.72, 24.76, 88.14%, 1.47, 0.42 and 0.28, respectively. The analysis of molecular variance revealed high genetic variation present within sampled geographical regions. Distance-based cluster analysis assigned the studied accessions into four clusters according to their geographical origin, which were also confirmed by principal coordinate analysis. In model-based Bayesian clustering, the maximum value of Δk was obtained when the collection of 118 assayed A. annua accessions assigned into two subgroups (K = 2). The results showed the high genetic variation in the collection of Iranian sweet wormwood which revealed by REMAP markers indicating the reliability and efficiency of this marker system for analysis of genetic diversity and population structure of A. annua.
Supplementary information: The online version contains supplementary material available at 10.1007/s40415-022-00860-x.
期刊介绍:
The Brazilian Journal of Botany is an international journal devoted to publishing a wide-range of research in plant sciences: biogeography, cytogenetics, ecology, economic botany, physiology and biochemistry, morphology and anatomy, molecular biology and diversity phycology, mycology, palynology, and systematics and phylogeny.
The journal considers for publications original articles, short communications, reviews, and letters to the editor.
Manuscripts describing new taxa based on morphological data only are suitable for submission; however information from multiple sources, such as ultrastructure, phytochemistry and molecular evidence are desirable.
Floristic inventories and checklists should include new and relevant information on other aspects, such as conservation strategies and biogeographic patterns.
The journal does not consider for publication submissions dealing exclusively with methods and protocols (including micropropagation) and biological activity of extracts with no detailed chemical analysis.