韩国一株新发现的鸡血清型肠炎沙门氏菌耐药基因磁带分析。

IF 4.7 2区 生物学 Q1 GENETICS & HEREDITY Mobile DNA Pub Date : 2023-04-24 DOI:10.1186/s13100-023-00292-8
Thanh Quang Tran, Minyoung Park, Jong Eun Lee, Soo Hyun Kim, Jae-Ho Jeong, Hyon E Choy
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引用次数: 2

摘要

抗微生物药物耐药性病原体是由于滥用抗微生物药物而造成的全球健康威胁。可通过移动遗传元件编码的抗性基因获得抗菌素耐药性。在这项研究中,我们从韩国感染的鸡中鉴定出一株肠炎血清型鸡沙门氏菌(SG4021),并通过全基因组测序鉴定了其质粒中存在抗性基因。然后将该序列与菌株SG_07Q015的质粒(P2)的序列进行比较,SG_07Q015是韩国分离的唯一具有基因组序列的菌株。结果显示,这两株菌株携带几乎相同的DNA,携带插入转座元件Tn21的整合子In2中的抗生素耐药基因盒,即对氨基糖苷具有抗性的aadA1耐药基因和对磺胺具有抗性的sul1耐药基因。有趣的是,尽管SG4021中存在sul1,但抗生素敏感性测试显示它对磺胺类药物敏感。进一步分析表明,这种差异是由于在SG4021中驱动sul1表达的启动子下游插入了约5 kb的ISCR16序列。利用不同的突变体,我们发现ISCR16的插入阻断了上游启动子sul1基因的表达。因此,抗菌素耐药性基因的功能决定了表型抗菌素耐药性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Analysis of antibiotic resistance gene cassettes in a newly identified Salmonella enterica serovar Gallinarum strain in Korea.

Antimicrobial resistant pathogens are a global health threat driven by the indiscriminate use of antimicrobials. Antimicrobial resistance can be acquired by resistance genes encoded by mobile genetic elements. In this study, we identified a strain of Salmonella enterica serovar Gallinarum (SG4021) from an infected chicken in Korea and characterized the presence of resistance genes in its plasmid by whole genome sequencing. The sequence was then compared with that of a plasmid (P2) from strain SG_07Q015, the only other strain of S. Gallinarum isolated in Korea for which a genome sequence is available. The results revealed that both strains harbored nearly identical DNA carrying antibiotic resistance gene cassettes inserted into integron In2 of the transposable element Tn21, namely an aadA1 resistance gene conferring resistance to aminoglycosides and a sul1 resistance gene conferring resistance to sulfonamide. Interestingly, despite the presence of sul1 in SG4021, an antibiotic sensitivity test revealed that it was sensitive to sulfonamides. Further analysis revealed that this disparity was due to the insertion of a ~ 5 kb ISCR16 sequence downstream of the promoter driving sul1 expression in SG4021. Using various mutants, we showed that the insertion of ISCR16 blocked the expression of the sul1 gene from the upstream promoter. Therefore, the functionality of antimicrobial resistance genes determines phenotypic antimicrobial resistance.

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来源期刊
Mobile DNA
Mobile DNA GENETICS & HEREDITY-
CiteScore
8.20
自引率
6.10%
发文量
26
审稿时长
11 weeks
期刊介绍: Mobile DNA is an online, peer-reviewed, open access journal that publishes articles providing novel insights into DNA rearrangements in all organisms, ranging from transposition and other types of recombination mechanisms to patterns and processes of mobile element and host genome evolution. In addition, the journal will consider articles on the utility of mobile genetic elements in biotechnological methods and protocols.
期刊最新文献
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