β- cd -牡荆素微球的制备及其对SW480细胞增殖的影响。

IF 2.8 4区医学 Q2 PHARMACOLOGY & PHARMACY Current drug delivery Pub Date : 2023-01-01 DOI:10.2174/1567201819666220825090426

Chengshi Ding, Shumeng Li, Deya Wang, Zhongjing Tian, Meiling Kang, Yingxia Zhang, Jing Ma, Yanmei Deng, Kai Zhang

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引用次数: 0

摘要

目的:为克服牡荆素体内不溶性和生物利用度低的问题，制备β-环糊精-牡荆素微球，观察其对SW480细胞增殖的影响。方法:采用扫描电镜、紫外光谱、傅里叶变换红外光谱、释放率分析等方法鉴定β- cd -牡荆素微球的形成。MTT法检测β- cd -牡荆素微球在6、12、24、48 h时对肿瘤细胞增殖的影响，荧光显微镜和流式细胞术观察β- cd -牡荆素微球对SW480细胞凋亡的影响。采用qPCR检测p53基因mRNA表达量。结果:成功制备了β- cd -牡荆素微球。0.1、0.2、0.4 mg/mL的β- cd -牡荆素微球均能抑制SW480细胞的增殖，且呈剂量依赖性和时间依赖性，其增殖抑制机制可能与p53基因表达上调导致细胞凋亡有关。结论:制备β- cd -牡荆素缓释微球是可行的，β- cd -牡荆素微球具有潜在的抗结直肠癌价值。

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Preparation of β-CD-Vitexin Microspheres and their Effects on SW480 Cell Proliferation.

Objective: In order to overcome the insolution and low bioavailability of the vitexin in vivo, β-cyclodextrin-vitexin (β-CD-vitexin) microspheres were prepared, and their effects on the proliferation of SW480 cells were observed.

Methods: Scanning electron microscopy, ultraviolet spectrum, Fourier transform infrared spectroscopy, and release rate analysis identified the formation of β-CD-vitexin microspheres. MTT assay detected the effect of β-CD-vitexin microspheres on tumor cell proliferation at 6, 12, 24, and 48 h. Fluorescence microscopy and flow cytometry were used to observe the effect of β-CD-vitexin microspheres on the apoptosis of SW480 cells. The mRNA expression of the p53 gene was measured by qPCR.

Results: β-CD-vitexin microspheres were successfully prepared. SW480 cell proliferation was inhibited by 0.1, 0.2, and 0.4 mg/mL of β-CD-vitexin microspheres in a dose- and time-dependent manner, and the mechanism of proliferation inhibition was related to cell apoptosis caused by the upregulated expression of p53 gene.

Conclusion: The preparation of β-CD-vitexin sustained release microspheres is feasible, and β-CDvitexin microspheres have potential anti-colorectal cancer value.

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来源期刊

Current drug delivery PHARMACOLOGY & PHARMACY-

CiteScore

5.10

自引率

4.20%

发文量

170

期刊介绍： Current Drug Delivery aims to publish peer-reviewed articles, research articles, short and in-depth reviews, and drug clinical trials studies in the rapidly developing field of drug delivery. Modern drug research aims to build delivery properties of a drug at the design phase, however in many cases this idea cannot be met and the development of delivery systems becomes as important as the development of the drugs themselves. The journal aims to cover the latest outstanding developments in drug and vaccine delivery employing physical, physico-chemical and chemical methods. The drugs include a wide range of bioactive compounds from simple pharmaceuticals to peptides, proteins, nucleotides, nucleosides and sugars. The journal will also report progress in the fields of transport routes and mechanisms including efflux proteins and multi-drug resistance. The journal is essential for all pharmaceutical scientists involved in drug design, development and delivery.