海洋酵母蚜虫Moesziomyces aphidis XM01高效生产甘露糖赤藓糖醇脂及其自组装纳米胶束的应用。

IF 5.8 2区 生物学 Q1 MARINE & FRESHWATER BIOLOGY Marine Life Science & Technology Pub Date : 2022-08-01 DOI:10.1007/s42995-022-00135-0
Guanshuo Yu, Xiaoxiang Wang, Chao Zhang, Zhe Chi, Zhenming Chi, Guanglei Liu
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引用次数: 3

摘要

甘露糖赤藓糖醇脂具有优异的物理化学性质、高的环境相容性和多种生物功能,是一种极具发展前景的生物表面活性剂。在这项研究中,我们鉴定了一株红树林酵母菌株Moesziomyces aphidis XM01,并将其用于高效的细胞外MEL生产。优化后的氮碳源为2.0 g/L NaNO3和70 g/L大豆油,7 d内MEL滴度达到64.5±0.7 g/L。在10 L的两段分批补料发酵过程中,8 d内MEL的最终滴度达到113.6±3.1 g/L,产量显著,分别为14.2 g·L-1·day-1和94.6 g/g(葡萄糖和豆油)。结构分析表明,产物主要为MEL-A,其脂肪酸谱仅由中链脂肪酸(c8 ~ c12)组成,其中以C10酸为主(77.81%)。进一步评价了该化合物作为一步自组装纳米胶束的应用。所得MEL纳米胶束具有良好的物理化学稳定性和抗菌活性。此外,以克拉霉素为模型疏水药物,MEL纳米胶束具有较高的负载能力,可用于低ph环境下的药物控制和持续释放。因此,蚜虫XM01是高效生产MEL的优良候选物,制备的MEL纳米胶束在制药和化妆品领域具有广阔的应用前景。补充信息:在线版本包含补充资料,下载地址:10.1007/s42995-022-00135-0。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Efficient production of mannosylerythritol lipids by a marine yeast Moesziomyces aphidis XM01 and their application as self-assembly nanomicelles.

Mannosylerythritol lipids (MELs) are one of the most promising biosurfactants because of their excellent physicochemical properties, high environmental compatibility, and various biological functions. In this study, a mangrove yeast strain Moesziomyces aphidis XM01 was identified and used for efficient extracellular MEL production. The MEL titer reached 64.5 ± 0.7 g/L at flask level within 7 days with the optimized nitrogen and carbon source of 2.0 g/L NaNO3 and 70 g/L soybean oil. Furthermore, during a 10-L two-stage fed-batch fermentation, the final MEL titer reached 113.6 ± 3.1 g/L within 8 days, with prominent productivity and yield of 14.2 g·L-1·day-1 and 94.6 g/g(glucose and soybean oil). Structural analysis indicated that the produced MELs were mainly MEL-A and its fatty acid profile was composed of only medium-chain fatty acids (C8-C12), especially C10 acids (77.81%). Further applications of this compound were evaluated as one-step self-assembly nanomicelles. The obtained MEL nanomicelles showed good physicochemical stability and antibacterial activity. In addition, using clarithromycin as a model hydrophobic drug, the MEL nanomicelles exhibited high loading capacity and could be used for the controlled and sustained drug release in low-pH environments. Therefore, M. aphidis XM01 is an excellent candidate for efficient MEL production, and the prepared MEL nanomicelles have broad application prospects in the pharmaceutical and cosmetic fields.

Supplementary information: The online version contains supplementary material available at 10.1007/s42995-022-00135-0.

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来源期刊
Marine Life Science & Technology
Marine Life Science & Technology MARINE & FRESHWATER BIOLOGY-
CiteScore
9.60
自引率
10.50%
发文量
58
期刊介绍: Marine Life Science & Technology (MLST), established in 2019, is dedicated to publishing original research papers that unveil new discoveries and theories spanning a wide spectrum of life sciences and technologies. This includes fundamental biology, fisheries science and technology, medicinal bioresources, food science, biotechnology, ecology, and environmental biology, with a particular focus on marine habitats. The journal is committed to nurturing synergistic interactions among these diverse disciplines, striving to advance multidisciplinary approaches within the scientific field. It caters to a readership comprising biological scientists, aquaculture researchers, marine technologists, biological oceanographers, and ecologists.
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