LINC00511编码的小肽LINC00511-133aa通过Wnt/β-catenin途径调节乳腺癌症细胞侵袭和干燥

IF 2.3 3区 生物学 Q3 BIOCHEMICAL RESEARCH METHODS Molecular and Cellular Probes Pub Date : 2023-06-01 DOI:10.1016/j.mcp.2023.101913
Zhongqiu Tan , Lifeng Zhao , Shiqing Huang , Qiulan Jiang , Yantao Wei , Junyun Long Wu , Zhiwen Zhang , Yepeng Li
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引用次数: 3

摘要

LINC00511是一种ncRNA的长链非编码RNA(lncRNA)。本研究旨在研究lncRNA LINC00511是否可以编码一种小肽LINC00511-133aa,以及该肽是否可以通过激活wnt/β-catenin途径促进癌症细胞转移和干燥。将LINC00511-133aa编码序列载体和对照载体转染到MCF-7和MDA-MB-231乳腺癌症细胞中,随后使用PCR、蛋白质印迹和免疫荧光测定评估肽表达。使用CCK8、凋亡、伤口愈合和transwell侵袭分析来评估细胞增殖、侵袭和凋亡,同时通过球体形成分析和干性标记物Oct4、Nanog和SOX2的蛋白质印迹分析来检测肿瘤干细胞的特征性变化。结果表明,LINC00511-133aa确实是由LINC00511编码的,并通过调节wnt/β-连环蛋白通路相关蛋白Bax、c-myc和CyclinD1的表达水平以及促进β-连环素蛋白进入细胞核来促进乳腺癌症细胞的侵袭性和干性,同时限制细胞凋亡。本研究为lncRNA LINC00511及其肽产物通过调节wnt/β-catenin途径参与乳腺癌症进展提供了证据。
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Small peptide LINC00511-133aa encoded by LINC00511 regulates breast cancer cell invasion and stemness through the Wnt/β-catenin pathway

LINC00511 is an long non-coding RNA (lncRNA) of ncRNAs,This study aimed to investigate whether the lncRNA LINC00511 could encode a small peptide, LINC00511-133aa, and whether this peptide could promote breast cancer cell metastasis and stemness by activating the wnt/β-catenin pathway. The LINC00511-133aa coding sequence vector and control vector were transfected into MCF-7 and MDA-MB-231 breast cancer cells, with subsequent assessment of peptide expression using PCR, western blotting, and immunofluorescence assays. Cell proliferation, invasion, and apoptosis were evaluated using CCK8, apoptotic, wound healing, and transwell invasion assays, while the characteristic changes of tumor stem cells were detected through sphere-forming assay and western blot analyses of the stemness markers Oct4, Nanog, and SOX2. Results showed that LINC00511-133aa was indeed encoded by LINC00511 and promoted the invasiveness and stemness of breast cancer cells while limiting apoptosis by modulating the expression levels of wnt/β-catenin pathway-related proteins Bax, c-myc, and CyclinD1, as well as facilitating β-catenin protein entry into the nucleus. This study provides evidence for the potential involvement of lncRNA LINC00511 and its peptide product in breast cancer progression via the regulation of the wnt/β-catenin pathway.

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来源期刊
Molecular and Cellular Probes
Molecular and Cellular Probes 生物-生化研究方法
CiteScore
6.80
自引率
0.00%
发文量
52
审稿时长
16 days
期刊介绍: MCP - Advancing biology through–omics and bioinformatic technologies wants to capture outcomes from the current revolution in molecular technologies and sciences. The journal has broadened its scope and embraces any high quality research papers, reviews and opinions in areas including, but not limited to, molecular biology, cell biology, biochemistry, immunology, physiology, epidemiology, ecology, virology, microbiology, parasitology, genetics, evolutionary biology, genomics (including metagenomics), bioinformatics, proteomics, metabolomics, glycomics, and lipidomics. Submissions with a technology-driven focus on understanding normal biological or disease processes as well as conceptual advances and paradigm shifts are particularly encouraged. The Editors welcome fundamental or applied research areas; pre-submission enquiries about advanced draft manuscripts are welcomed. Top quality research and manuscripts will be fast-tracked.
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