短期、长期和间歇性大肠杆菌感染对雄性C57BL/6J小鼠前列腺组织学和泌尿生理的影响。

IF 1.5 Q3 UROLOGY & NEPHROLOGY American journal of clinical and experimental urology Pub Date : 2023-01-01
Hannah Ruetten, Simran K Sandhu, Olivia Fox, Jonathan Zhu, Jaskiran K Sandhu, Chad M Vezina
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引用次数: 0

摘要

前列腺炎症和前列腺纤维化与男性下尿路功能障碍有关。经尿道尿路致病性大肠杆菌感染引起的前列腺炎症足以增加雄性小鼠前列腺胶原蛋白含量。目前尚不清楚前列腺感染的顺序、持续时间和时间是否以及如何影响泌尿功能、前列腺炎症和胶原蛋白含量。我们在研究终点前两周(评估感染的短期影响)、研究终点前10周(评估感染的长期影响)或研究终点前2周、6周和10周(评估反复间歇性感染的影响)将经尿道导管置入成年雄性C57BL/6J小鼠中,以输送尿路致病性大肠杆菌UTI189。所有实验组小鼠插管次数相同,未灌注大肠杆菌时灌注无菌生理盐水,以控制插管变量。我们测量了自由捕获尿液中的细菌载量、体重和膀胱和前列腺背部的重量;前列腺白细胞密度,胶原蛋白和原胶原蛋白1A1产生细胞,和泌尿功能。经尿道输注大肠杆菌对有一次或多次经尿道输注无菌生理盐水或大肠杆菌病史的小鼠造成更严重和持续的细菌尿。反复间歇性感染导致膀胱相对湿重大于单次感染。然而,通过空斑试验测量的排尿功能以及前列腺背侧组织切片中胶原蛋白和ProCOL1A1+细胞的密度在感染组之间没有显著差异。与未感染的对照组相比,在研究终点前两周感染的小鼠前列腺背侧的CD45+白细胞密度更高,但在其他感染组中没有。
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The impact of short term, long term and intermittent E. coli infection on male C57BL/6J mouse prostate histology and urinary physiology.

Prostatic inflammation and prostatic fibrosis are associated with lower urinary tract dysfunction in men. Prostatic inflammation arising from a transurethral uropathogenic E. coli infection is sufficient to increase prostatic collagen content in male mice. It is not known whether and how the sequence, duration and chronology of prostatic infection influence urinary function, prostatic inflammation and collagen content. We placed a transurethral catheter into adult male C57BL/6J mice to deliver uropathogenic E. coli UTI189 two-weeks prior to study endpoint (to evaluate the short-term impact of infection), 10-weeks prior to study endpoint (to evaluate the long-term impact of infection), or two-, six-, and ten-weeks prior to endpoint (to evaluate the impact of repeated intermittent infection). Mice were catheterized the same number of times across all experimental groups and instilled with sterile saline when not instilled with E. coli to control for the variable of catheterization. We measured bacterial load in free catch urine, body weight and weight of bladder and dorsal prostate; prostatic density of leukocytes, collagen and procollagen 1A1 producing cells, and urinary function. Transurethral E. coli instillation caused more severe and persistent bacteriuria in mice with a history of one or more transurethral instillations of sterile saline or E. coli. Repeated intermittent infections resulted in a greater relative bladder wet weight than single infections. However, voiding function, as measured by the void spot assay, and the density of collagen and ProCOL1A1+ cells in dorsal prostate tissue sections did not significantly differ among infection groups. The density of CD45+ leukocytes was greater in the dorsal prostate of mice infected two weeks prior to study endpoint but not in other infection groups compared to uninfected controls.

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