非血液学肿瘤血源性细胞学制备的特性规程。

IF 1.6 4区 医学 Q3 PATHOLOGY Acta Cytologica Pub Date : 2023-01-01 DOI:10.1159/000527904
Natalia Malara, Giuseppe Donato, Francesca Ferrazzo, Nastassia Carmelina Garo, Franco Fulciniti
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引用次数: 1

摘要

液体活检,特别是通过从外周血中分离、扩增和检查循环肿瘤细胞(ctc)来进行的活检,已经成为临床肿瘤学中一种创新和变革的诊断工具。ctc已经进入临床实践,作为浸润性肿瘤活检的替代方法,用于检测术后和/或治疗后的微小残留疾病,预测癌症复发和实时治疗反应。在这种情况下,回顾性观察项目,被称为characterx,已经允许声明,有可能利用血液为基础的细胞学样本,通过短期培养和体外CTC扩增。方法:该方法首先基于梯度沉降技术,在不完全剥夺血液细胞样本的情况下进行贫困化,然后进行短期(14天)的体外培养和扩增以及细胞形态学和流式细胞术分析,以研究扩增后的细胞群是否具有增殖优势并符合标准,这与已知的原发肿瘤一致。结果:该方法的独创性在于,除了上述暴露标准外,从外周血中分离细胞没有选择偏差(如免疫磁珠治疗或初步免疫细胞化学),这可能会给评估的细胞群带来一些限制。结论:通过这种方法获得的扩增细胞群的检查对病理学家和临床医生都是非常有益的,病理学家可以评估多种肿瘤相关变量(如免疫细胞化学,几个参数的流式细胞术,以及从中获得的细胞悬液和细胞块的分子病理学)。
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The Charactex Protocol for Blood-Derived Cytological Preparation of Nonhematological Cancer.

Introduction: Liquid biopsy, especially when performed by the isolation, expansion, and examination of circulating tumor cells (CTCs) from peripheral blood, has become an innovative and transforming diagnostic tool in Clinical Oncology. The CTCs have already entered the clinical practice as an alternative method to invasive tumor biopsy for detecting postsurgical and/or posttreatment minimal residual disease, to predict cancer recurrence and real-time treatment response. In this context, the retrospective observational project, known as CHARACTEX, has permitted to state that it is possible to exploit blood-based cytologic samples through short-term culture and in vitro CTC expansion.

Methods: This method is based initially on a gradient-sedimentation technique, which impoverishes without completely depriving the obtained sample from the hematological cells, followed by short-term (14 days) in vitro culture and expansion and cytomorphological and flow cytometric analysis to investigate whether the expanded cell population possesses proliferative advantage and fits with criteria, which are consistent to the known primary tumor.

Results: The originality of this method is that, apart from the above exposed criteria, there is no selection bias for the isolation of the cells from peripheral blood (like immunomagnetic bead treatment or preliminary immunocytochemistry), which can potentially introduce some limitation to the cell population under evaluation.

Conclusion: The examination of the expanded cell population obtained by this method is very rewarding for both the pathologist - who can assess multiple tumor-related variables (like immunocytochemistry, flow cytometry of several parameters, and molecular pathology on cell suspensions and cell blocks obtained from them) - and the clinician.

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来源期刊
Acta Cytologica
Acta Cytologica 生物-病理学
CiteScore
3.70
自引率
11.10%
发文量
46
审稿时长
4-8 weeks
期刊介绍: With articles offering an excellent balance between clinical cytology and cytopathology, ''Acta Cytologica'' fosters the understanding of the pathogenetic mechanisms behind cytomorphology and thus facilitates the translation of frontline research into clinical practice. As the official journal of the International Academy of Cytology and affiliated to over 50 national cytology societies around the world, ''Acta Cytologica'' evaluates new and existing diagnostic applications of scientific advances as well as their clinical correlations. Original papers, review articles, meta-analyses, novel insights from clinical practice, and letters to the editor cover topics from diagnostic cytopathology, gynecologic and non-gynecologic cytopathology to fine needle aspiration, molecular techniques and their diagnostic applications. As the perfect reference for practical use, ''Acta Cytologica'' addresses a multidisciplinary audience practicing clinical cytopathology, cell biology, oncology, interventional radiology, otorhinolaryngology, gastroenterology, urology, pulmonology and preventive medicine.
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