澄清CRISPR:为什么在人类基因组中发现的重复序列不应该被认为是CRISPR。

IF 3.7 4区生物学 Q2 GENETICS & HEREDITY CRISPR Journal Pub Date : 2023-06-01 Epub Date: 2023-04-11 DOI:10.1089/crispr.2022.0106

Murat Buyukyoruk, William S Henriques, Blake Wiedenheft

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引用次数: 1

摘要

聚集规律间隔短回文重复序列(crispr)及其相关基因(cas)是保护细菌和古细菌免受病毒感染的适应性免疫系统的重要组成部分。在大约40%的细菌和85%的古细菌基因组中发现了CRISPR-Cas系统，但在真核生物基因组中没有发现。最近，发表在《通讯生物学》上的一篇文章报道称，在人类基因组中发现了12572个假定的crispr，他们称之为“hCRISPR”。在这项研究中，我们试图重现这一分析，并表明在人类基因组中被鉴定为假定的CRISPR位点的重复元件既不包含重复-间隔-重复结构，也不包含功能性CRISPR系统所具有的cas基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Clarifying CRISPR: Why Repeats Identified in the Human Genome Should Not Be Considered CRISPRs.

Clustered regularly interspaced short palindromic repeats (CRISPRs) and their associated genes (cas) are essential components of adaptive immune systems that protect bacteria and archaea from viral infection. CRISPR-Cas systems are found in about 40% of bacterial and 85% of archaeal genomes, but not in eukaryotic genomes. Recently, an article published in Communications Biology reported the identification of 12,572 putative CRISPRs in the human genome, which they call "hCRISPR." In this study, we attempt to reproduce this analysis and show that repetitive elements identified as putative CRISPR loci in the human genome contain neither the repeat-spacer-repeat architecture nor the cas genes characteristic of functional CRISPR systems.

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来源期刊

CRISPR Journal Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.30

自引率

2.70%

发文量

期刊介绍： In recognition of this extraordinary scientific and technological era, Mary Ann Liebert, Inc., publishers recently announced the creation of The CRISPR Journal -- an international, multidisciplinary peer-reviewed journal publishing outstanding research on the myriad applications and underlying technology of CRISPR. Debuting in 2018, The CRISPR Journal will be published online and in print with flexible open access options, providing a high-profile venue for groundbreaking research, as well as lively and provocative commentary, analysis, and debate. The CRISPR Journal adds an exciting and dynamic component to the Mary Ann Liebert, Inc. portfolio, which includes GEN (Genetic Engineering & Biotechnology News) and more than 80 leading peer-reviewed journals.