减少细菌基因组以优化合成生物学底盘:综述。

IF 8.1 2区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Critical Reviews in Biotechnology Pub Date : 2024-06-01 Epub Date: 2023-06-28 DOI:10.1080/07388551.2023.2208285
Shuai Ma, Tianyuan Su, Xuemei Lu, Qingsheng Qi
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引用次数: 0

摘要

具有精简基因组的细菌含有基本代谢网络的全部功能基因,能够更有效地合成所需的产品,因此在工业应用中具有作为生产平台的优势。为了获得精简的底盘基因组,人们做了大量工作来减少现有的细菌基因组。这项工作分为两类:合理缩减和随机缩减。过去几十年来,基本基因组的确定和各种基因组删除技术的出现极大地促进了许多细菌基因组的缩减。一些构建的基因组具有工业应用的理想特性,如:提高基因组稳定性、转化能力、细胞生长和生物材料生产率。一些基因组还原菌株的生长能力下降、生理表型紊乱,可能会限制它们作为优化细胞工厂的应用。本综述评估了迄今为止在细菌基因组还原以构建合成生物学最佳底盘方面取得的进展,包括:重要基因组的鉴定、基因组缺失技术、人工精简基因组的特性和工业应用、构建还原基因组过程中遇到的障碍以及未来展望。
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Bacterial genome reduction for optimal chassis of synthetic biology: a review.

Bacteria with streamlined genomes, that harbor full functional genes for essential metabolic networks, are able to synthesize the desired products more effectively and thus have advantages as production platforms in industrial applications. To obtain streamlined chassis genomes, a large amount of effort has been made to reduce existing bacterial genomes. This work falls into two categories: rational and random reduction. The identification of essential gene sets and the emergence of various genome-deletion techniques have greatly promoted genome reduction in many bacteria over the past few decades. Some of the constructed genomes possessed desirable properties for industrial applications, such as: increased genome stability, transformation capacity, cell growth, and biomaterial productivity. The decreased growth and perturbations in physiological phenotype of some genome-reduced strains may limit their applications as optimized cell factories. This review presents an assessment of the advancements made to date in bacterial genome reduction to construct optimal chassis for synthetic biology, including: the identification of essential gene sets, the genome-deletion techniques, the properties and industrial applications of artificially streamlined genomes, the obstacles encountered in constructing reduced genomes, and the future perspectives.

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来源期刊
Critical Reviews in Biotechnology
Critical Reviews in Biotechnology 工程技术-生物工程与应用微生物
CiteScore
20.80
自引率
1.10%
发文量
71
审稿时长
4.8 months
期刊介绍: Biotechnological techniques, from fermentation to genetic manipulation, have become increasingly relevant to the food and beverage, fuel production, chemical and pharmaceutical, and waste management industries. Consequently, academic as well as industrial institutions need to keep abreast of the concepts, data, and methodologies evolved by continuing research. This journal provides a forum of critical evaluation of recent and current publications and, periodically, for state-of-the-art reports from various geographic areas around the world. Contributing authors are recognized experts in their fields, and each article is reviewed by an objective expert to ensure accuracy and objectivity of the presentation.
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