引起太子黑腐病的小双plodia和大黄双plodia的鉴定与鉴定。

IF 1.8 3区 农林科学 Q2 PLANT SCIENCES Plant Pathology Journal Pub Date : 2023-06-01 DOI:10.5423/PPJ.OA.03.2023.0039
Sungmun Kwon, Jungyeon Kim, Younmi Lee, Kotnala Balaraju, Yongho Jeon
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引用次数: 0

摘要

在韩国庆尚北道永川市发现了从感染的中国榅桲树上分离出的真菌引起的黑腐病。木瓜的叶子枯萎了,变成了红褐色,果实变成了黑色的木乃伊。为了阐明这些症状的原因,在马铃薯葡萄糖琼脂和Levan培养基上从受感染的叶片和果实组织中分离出病原菌。几个真菌菌落形成蓬松的白色或深灰色菌丝,两种真菌形成空中白色菌丝,在边缘广泛生长。显微镜下观察,研究真菌在不同培养基上的生长特性,并利用内部转录间隔物、β-微管蛋白和翻译伸长因子1-α基因进行分子鉴定。病原真菌鉴定为细小双plodia parva和双plodia crataegicola。致病性试验表明,接种病菌的果实呈层状,呈褐腐;叶片呈圆形棕色坏死灶。出现的症状与现场观察到的症状相似。真菌病原体被重新分离,以满足科赫的假设。用真菌病原菌对苹果进行接种,研究其寄主范围。果实致病性强,接种后3 d出现褐变和腐烂症状。为了确定病原菌的防治效果,用4种已登记的杀菌剂进行了杀真菌敏感性试验。噻吩酸甲酯、丙泊尼和戊康唑抑制病原菌菌丝生长。据我们所知,这是国内首次从引起黑腐病的中国榅桲果实和叶片中分离鉴定出真菌病原菌D. parva和D. crataegicola。
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Identification and Characterization of Diplodia parva and Diplodia crataegicola Causing Black Rot of Chinese Quince.

Fungal isolates from infected Chinese quince trees were found to cause black rot in Yeongcheon, Gyeongsangbuk Province, Korea. The quince leaves withered and turned reddish-brown and fruits underwent black mummification. To elucidate the cause of these symptoms, the pathogen was isolated from infected leaf and fruit tissues on potato dextrose agar and Levan media. Several fungal colonies forming a fluffy white or dark gray mycelium and two types of fungi forming an aerial white mycelium, growing widely at the edges, were isolated. Microscopic observations, investigation of fungal growth characteristics on various media, and molecular identification using an internal transcribed spacer, β-tubulin, and translation elongation factor 1-α genes were performed. The fungal pathogens were identified as Diplodia parva and Diplodia crataegicola. Pathogenicity tests revealed that the pathogen-inoculated fruits exhibited a layered pattern, turning brown rotting; leaves showed circular brown necrotic lesions. The developed symptoms were similar to those observed in the field. Fungal pathogens were reisolated to fulfill Koch's postulates. Apples were inoculated with fungal pathogens to investigate the host range. Strong pathogenicity was evident in the fruits, with browning and rotting symptoms 3 days after inoculation. To determine pathogen control, a fungicidal sensitivity test was conducted using four registered fungicides. Thiophanate-methyl, propineb, and tebuconazole inhibited the mycelial growth of pathogens. To the best of our knowledge, this is the first report on the isolation and identification of the fungal pathogens D. parva and D. crataegicola from infected fruits and leaves of Chinese quince, causing black rot disease in Korea.

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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
期刊最新文献
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