作为骨组织再生支架的生物活性醋酸纤维素电纺垫。

IF 3 Q3 MATERIALS SCIENCE, BIOMATERIALS International Journal of Biomaterials Pub Date : 2022-02-04 eCollection Date: 2022-01-01 DOI:10.1155/2022/3255039
Simara Laboy-López, Pedro O Méndez Fernández, Jorge G Padilla-Zayas, Eduardo Nicolau
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摘要

过去几十年来,基于细胞的骨组织工程(BTE)方法一直依赖于使用无法复制骨微环境复杂性的模型。为了满足对可模拟骨细胞外基质(ECM)并有助于骨组织再生(BTR)的可行材料的需求,有关支架开发的研究一直在进行。在这项工作中,利用电纺丝技术开发了一种多孔醋酸纤维素(CA)纤维毡,并对纤维毡进行了化学改性,使其表面具有生物活性,促进骨传导和骨诱导。利用傅立叶变换红外光谱和扫描电镜/电子显微镜对纤维毡进行了表征,以验证化学修饰并评估其结构完整性。通过耦合粘附肽 KRSR、RGD 和生长因子 BMP-2,纤维毡被生物活化,并通过免疫细胞化学(ICC)技术评估了其诱导的生物反应,以研究过早成骨细胞(hFOB 1.19)的粘附、增殖和分化。生物学评估显示,在 48 小时和 7 天的短期培养中,多肽的存在对增殖和粘附有显著影响,而在 14 天的长期培养中,多肽对成骨祖细胞的分化和成熟没有显著影响。根据所获得的结果,可以得出结论:CA 多孔纤维毡提供了一种很有前景的表面形态,既具有生物相容性,又能在添加成骨肽后增强生物活性,从而促进骨传导,形成新的组织。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Bioactive Cellulose Acetate Electrospun Mats as Scaffolds for Bone Tissue Regeneration.

In the last decades, cell-based approaches for bone tissue engineering (BTE) have relied on using models that cannot replicate the complexity of the bone microenvironment. There is an ongoing amount of research on scaffold development responding to the need for feasible materials that can mimic the bone extracellular matrix (ECM) and aid bone tissue regeneration (BTR). In this work, a porous cellulose acetate (CA) fiber mat was developed using the electrospinning technique and the mats were chemically modified to bioactivate their surface and promote osteoconduction and osteoinduction. The mats were characterized using FTIR and SEM/EDS to validate the chemical modifications and assess their structural integrity. By coupling adhesive peptides KRSR, RGD, and growth factor BMP-2, the fiber mats were bioactivated, and their induced biological responses were evaluated by employing immunocytochemical (ICC) techniques to study the adhesion, proliferation, and differentiation of premature osteoblast cells (hFOB 1.19). The biological assessment revealed that at short culturing periods of 48 hours and 7 days, the presence of the peptides was significant for proliferation and adhesion, whereas at longer culture times of 14 days, it had no significant effect on differentiation and maturation of the osteogenic progenitor cells. Based on the obtained results, it is thus concluded that the CA porous fiber mats provide a promising surface morphology that is both biocompatible and can be rendered bioactive upon the addition of osteogenic peptides to favor osteoconduction leading to new tissue formation.

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来源期刊
International Journal of Biomaterials
International Journal of Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
4.30
自引率
3.20%
发文量
50
审稿时长
21 weeks
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