利用山裸孢菌合成金纳米粒子及其生物学特性:先驱报告。

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal, genetic engineering & biotechnology Pub Date : 2023-06-26 DOI:10.1186/s43141-023-00525-6
Krishnakumari N Patel, Pooja G Trivedi, Milan S Thakar, Kush V Prajapati, Dhruv K Prajapati, Gaurang M Sindhav
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引用次数: 0

摘要

背景:十亿分之一米或更小尺寸的材料的形成、混合和表征被称为纳米技术。本研究的目的是从山裸孢菌叶提取物中合成生态友好的金纳米粒子(AuNPs),对其进行表征,评估其与不同类型脱氧核糖核酸(DNA)的相互作用,并研究其抗氧化和毒性。结果:通过从黄色到红粉色的颜色变化以及使用紫外-可见分光光度计验证了生物合成的AuNPs的存在。傅立叶变换红外光谱分析表明,存在负责AuNPs还原的植物成分,如醇类、酚类和硝基化合物。Zeta粒度仪和559.6d.nm的ζ电位 - 分别为4.5 mV,显示出电位稳定性。平均尺寸在10和50nm之间,X射线衍射(XRD)和高分辨率透射电子显微镜(HR-TEM)揭示了AuNPs的结晶形成。在原子力显微镜(AFM)的帮助下,确定了具有3D表征、不规则球形和6.48nm尺寸的AuNPs的表面拓扑结构。通过场发射扫描电子显微镜(FESEM)研究,发现AuNPs具有一些不规则和球形形状,尺寸在2到20nm之间。当用小牛胸腺DNA(CT-DNA)和鲱鱼精子DNA(HS-DNA)测试AuNPs的生物利用度时,光谱中的变化是可见的。此外,DNA缺口分析与pBR322 DNA的相互作用证实了其理化性质和抗氧化性质。通过使用2,2-二苯基-1-苦基肼(DPPH)测定也发现了同样的情况,其显示出70-80%的抑制率。最后,3-(4,5-二甲基噻唑-2-基)-2,5-二苯基-2H-溴化四唑啉(MTT)分析显示,MCF-7细胞系的活力随着剂量的增加而降低,从77.74%降至46.99%。结论:通过生物过程合成AuNPs并首次采用蒙塔纳G.揭示了潜在的DNA相互作用、抗氧化和细胞毒性能力。因此,在治疗领域以及其他领域开辟了新的可能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Gold nanoparticles synthesis using Gymnosporia montana L. and its biological profile: a pioneer report.

Background: The forming, blending, and characterization of materials at a size of one billionth of a meter or less is referred to as nanotechnology. The objective of the current study was to synthesize ecologically friendly gold nanoparticles (AuNPs) from Gymnosporia montana L. (G. montana) leaf extract, characterize them, assess their interaction with different types of deoxyribonucleic acid (DNA), and investigate their antioxidant and toxic capabilities.

Results: The biosynthesized AuNPs presence was validated by a color change from yellow to reddish pink as well as using UV-visible spectrophotometer. Fourier transform infrared (FTIR) spectroscopy analysis showed the presence of phytoconstituents like, alcohols, phenols, and nitro compounds responsible for the reduction of AuNPs. Zeta sizer and zeta potential of 559.6 d. nm and - 4.5 mV, respectively, demonstrated potential stability. With an average size between 10 and 50 nm, X-ray diffraction (XRD), and high-resolution transmission electron microscope (HR-TEM), revealed the crystalline formation of AuNPs. Surface topology with 3D characterization, irregular spherical shape, and size with 6.48 nm of AuNPs was determined with the help of an atomic force microscope (AFM). AuNPs with some irregular and spherical shapes, and sizes between 2 and 20 nm, were revealed by field emission scanning electron microscope (FESEM) investigation. Shifts in the spectrum were visible when the bioavailability of AuNPs with calf-thymus DNA (CT-DNA) and Herring sperm DNA (HS-DNA) was tested. Additionally, the DNA nicking assay's interaction with pBR322 DNA confirmed its physiochemical and antioxidant properties. The same was also found by using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, which showed a 70-80% inhibition rate. Finally, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay revealed that viability decreased with increasing dosage, going from 77.74 to 46.99% on MCF-7 cell line.

Conclusion: Synthesizing AuNPs through biogenic processes and adopting G. montana for the first time revealed potential DNA interaction, antioxidant, and cytotoxicity capabilities. Thus, opening new possibilities in the turf of therapeutics as well as in other areas.

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