在日本分离的针叶树苗和块根菌丝体菌株之间通过离体合成形成的外生菌根的形态特征。

IF 1.5 4区 生物学 Q4 MYCOLOGY Mycoscience Pub Date : 2022-01-20 eCollection Date: 2022-01-01 DOI:10.47371/mycosci.2021.11.002
Keisuke Obase
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摘要

在体外将松树(Pinus densiflora)和沙棘(Abies sachalinensis)的幼苗接种到 Puberulum 支系的块菌菌丝株上,以研究其外生菌根的形态特征。将轴向发芽的幼苗接种到 Puberulum 支系五个类群的菌丝体中,并在玻璃罐中的光照培养箱中培养 4 个月。通过对合成的外生菌根进行核糖体内部转录间隔条形编码,证实接种的块根菌株成功地在幼苗上定植。这些外生菌根的特征是淡黄色至棕色、短针状子囊菌、网状菌丝排列以及菌盖表面的表皮细胞;值得注意的是,这些特征与 Puberulum 各支系成员的外生菌根相似。由于不同块根菌种的外生菌根在形态特征上无法区分,因此有必要采用分子技术来鉴定块根菌支系中块根菌种形成的外生菌根。
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Morphological characteristics of ectomycorrhizas formed by in vitro synthesis between conifer seedlings and Tuber mycelial strains of the Puberulum clade isolated in Japan.

Seedlings of Pinus densiflora and Abies sachalinensis were inoculated with Tuber mycelial strains of the Puberulum clade in vitro to examine the morphological characteristics of their ectomycorrhizas. Axenically germinated seedlings were inoculated with the mycelia of five taxa from the Puberulum clade and grown in glass jars for 4 mo in an illuminated incubator. The seedlings were successfully colonized by the inoculated Tuber strains, as confirmed by the nuclear ribosomal internal transcribed spacer barcoding of the synthesized ectomycorrhizas. The ectomycorrhizas were characterized by a pale yellow to brown color, short needle-shaped cystidia, and net-like hyphal arrangement, and epidermoid cells on the mantle surface; notably, these features are similar to the ectomycorrhizas of various Puberulum clade members. As the ectomycorrhizas of different Tuber species are indistinguishable by morphological characters, molecular techniques are necessary to identify ectomycorrhizas formed by Tuber species within the Puberulum clade.

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来源期刊
Mycoscience
Mycoscience MYCOLOGY-
CiteScore
3.00
自引率
7.10%
发文量
32
审稿时长
3 months
期刊介绍: Mycoscience is the official English-language journal of the Mycological Society of Japan and is issued bimonthly. Mycoscience publishes original research articles and reviews on various topics related to fungi including yeasts and other organisms that have traditionally been studied by mycologists. The research areas covered by Mycoscience extend from such purely scientific fields as systematics, evolution, phylogeny, morphology, ecology, physiology, biochemistry, genetics, and molecular biology, to agricultural, medical, and industrial applications. New and improved applications of well-established mycological techniques and methods are also covered.
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