转录组分析揭示了Zelda基因在木犀草胚胎和翅膀发育调控中的作用。

IF 1.6 3区 农林科学 Q2 ENTOMOLOGY Bulletin of Entomological Research Pub Date : 2023-10-01 Epub Date: 2023-07-21 DOI:10.1017/S0007485323000263
Shanshan Gao, Shuang Xue, Tian Gao, Ruixue Lu, Xinyi Zhang, Yonglei Zhang, Kunpeng Zhang, Ruimin Li
{"title":"转录组分析揭示了Zelda基因在木犀草胚胎和翅膀发育调控中的作用。","authors":"Shanshan Gao, Shuang Xue, Tian Gao, Ruixue Lu, Xinyi Zhang, Yonglei Zhang, Kunpeng Zhang, Ruimin Li","doi":"10.1017/S0007485323000263","DOIUrl":null,"url":null,"abstract":"<p><p>Zinc finger protein (Zelda) of <i>Tribolium castaneum</i> (<i>TcZelda</i>) has been showed to play pivotal roles in embryonic development and metamorphosis. However, the regulatory mechanism of <i>TcZelda</i> associated with these physiology processes is unclear. Herein, the developmental expression profile showed that <i>Zelda</i> of <i>T. castaneum</i> was highly expressed in early eggs. Tissue expression profiling revealed that <i>TcZelda</i> was mainly expressed in the larval head and adult ovary of late adults and late larvae. <i>TcZelda</i> knockdown led to a 95% mortality rate in adults. These results suggested that <i>TcZelda</i> is related to the activation of the zygote genome in early embryonic development. Furthermore, 592 differentially expressed genes were identified from the ds<i>Zelda</i> treated group. Compared with the control group, <i>altered disjunction</i> (<i>ALD</i>) and <i>AGAP005368-PA</i> (<i>GAP</i>) in the ds<i>Zelda</i> group were significantly down-regulated, while <i>TGF-beta, propeptide</i> (<i>TGF</i>) was significantly up-regulated, suggesting that <i>TcZelda</i> may be involved in insect embryonic development. In addition, the expression of <i>Ubx ultrabithorax</i> (<i>UBX</i>), <i>Cx cephalothorax</i> (<i>CX</i>), <i>En engrailed</i> (<i>EN</i>), and two <i>Endocuticle structural glycoprotein sgabd</i> (<i>ABD</i>) genes were significantly down-regulated, suggesting that they may cooperate with <i>TcZelda</i> to regulate the development of insect wings. Additionally, <i>Elongation</i> (<i>ELO</i>), <i>fatty acid synthase</i> (<i>FAS</i>), and <i>fatty acyl-CoA desaturase</i> (<i>FAD</i>) expression was inhibited in ds<i>Zelda</i> insects, which could disturb the lipase signaling pathways, thus, disrupting the insect reproductive system and pheromone synthesis. These results may help reveal the function of <i>TcZelda</i> in insects and the role of certain genes in the gene regulatory network and provide new ideas for the prevention and control of <i>T. castaneum</i>.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"587-597"},"PeriodicalIF":1.6000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Transcriptome analysis reveals the role of Zelda in the regulation of embryonic and wing development of <i>Tribolium castaneum</i>.\",\"authors\":\"Shanshan Gao, Shuang Xue, Tian Gao, Ruixue Lu, Xinyi Zhang, Yonglei Zhang, Kunpeng Zhang, Ruimin Li\",\"doi\":\"10.1017/S0007485323000263\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Zinc finger protein (Zelda) of <i>Tribolium castaneum</i> (<i>TcZelda</i>) has been showed to play pivotal roles in embryonic development and metamorphosis. However, the regulatory mechanism of <i>TcZelda</i> associated with these physiology processes is unclear. Herein, the developmental expression profile showed that <i>Zelda</i> of <i>T. castaneum</i> was highly expressed in early eggs. Tissue expression profiling revealed that <i>TcZelda</i> was mainly expressed in the larval head and adult ovary of late adults and late larvae. <i>TcZelda</i> knockdown led to a 95% mortality rate in adults. These results suggested that <i>TcZelda</i> is related to the activation of the zygote genome in early embryonic development. Furthermore, 592 differentially expressed genes were identified from the ds<i>Zelda</i> treated group. Compared with the control group, <i>altered disjunction</i> (<i>ALD</i>) and <i>AGAP005368-PA</i> (<i>GAP</i>) in the ds<i>Zelda</i> group were significantly down-regulated, while <i>TGF-beta, propeptide</i> (<i>TGF</i>) was significantly up-regulated, suggesting that <i>TcZelda</i> may be involved in insect embryonic development. In addition, the expression of <i>Ubx ultrabithorax</i> (<i>UBX</i>), <i>Cx cephalothorax</i> (<i>CX</i>), <i>En engrailed</i> (<i>EN</i>), and two <i>Endocuticle structural glycoprotein sgabd</i> (<i>ABD</i>) genes were significantly down-regulated, suggesting that they may cooperate with <i>TcZelda</i> to regulate the development of insect wings. Additionally, <i>Elongation</i> (<i>ELO</i>), <i>fatty acid synthase</i> (<i>FAS</i>), and <i>fatty acyl-CoA desaturase</i> (<i>FAD</i>) expression was inhibited in ds<i>Zelda</i> insects, which could disturb the lipase signaling pathways, thus, disrupting the insect reproductive system and pheromone synthesis. These results may help reveal the function of <i>TcZelda</i> in insects and the role of certain genes in the gene regulatory network and provide new ideas for the prevention and control of <i>T. castaneum</i>.</p>\",\"PeriodicalId\":9370,\"journal\":{\"name\":\"Bulletin of Entomological Research\",\"volume\":\" \",\"pages\":\"587-597\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2023-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bulletin of Entomological Research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1017/S0007485323000263\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/7/21 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Entomological Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1017/S0007485323000263","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/7/21 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

锌指蛋白(Zinc finger protein, Zelda)已被证实在castaneum (Tribolium castaneum, TcZelda)的胚胎发育和变态过程中起关键作用。然而,TcZelda与这些生理过程相关的调控机制尚不清楚。在此,发育表达谱显示,castaneum的Zelda在早期卵中高度表达。组织表达谱分析显示,TcZelda主要表达于晚期成虫和晚期幼虫的幼虫头部和成虫卵巢。TcZelda基因敲低导致成人95%的死亡率。这些结果表明,TcZelda与胚胎早期发育过程中受精卵基因组的激活有关。此外,从dsZelda处理组中鉴定出592个差异表达基因。与对照组相比,dsZelda组的改变断开(ALD)和AGAP005368-PA (GAP)显著下调,TGF- β、前肽(TGF)显著上调,提示TcZelda可能参与了昆虫胚胎发育。此外,Ubx超双胸(Ubx)、Cx头胸(Cx)、En enengrailed (En)和两个endocticle structural glycoprotein sgabd (ABD)基因的表达均显著下调,提示它们可能与TcZelda协同调控昆虫翅膀的发育。此外,延长酶(ELO)、脂肪酸合成酶(FAS)和脂肪酸酰基辅酶a去饱和酶(FAD)在dsZelda昆虫中的表达受到抑制,这可能会干扰脂肪酶信号通路,从而破坏昆虫生殖系统和信息素的合成。这些结果可能有助于揭示TcZelda在昆虫体内的功能和部分基因在基因调控网络中的作用,并为castaneum的防治提供新的思路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Transcriptome analysis reveals the role of Zelda in the regulation of embryonic and wing development of Tribolium castaneum.

Zinc finger protein (Zelda) of Tribolium castaneum (TcZelda) has been showed to play pivotal roles in embryonic development and metamorphosis. However, the regulatory mechanism of TcZelda associated with these physiology processes is unclear. Herein, the developmental expression profile showed that Zelda of T. castaneum was highly expressed in early eggs. Tissue expression profiling revealed that TcZelda was mainly expressed in the larval head and adult ovary of late adults and late larvae. TcZelda knockdown led to a 95% mortality rate in adults. These results suggested that TcZelda is related to the activation of the zygote genome in early embryonic development. Furthermore, 592 differentially expressed genes were identified from the dsZelda treated group. Compared with the control group, altered disjunction (ALD) and AGAP005368-PA (GAP) in the dsZelda group were significantly down-regulated, while TGF-beta, propeptide (TGF) was significantly up-regulated, suggesting that TcZelda may be involved in insect embryonic development. In addition, the expression of Ubx ultrabithorax (UBX), Cx cephalothorax (CX), En engrailed (EN), and two Endocuticle structural glycoprotein sgabd (ABD) genes were significantly down-regulated, suggesting that they may cooperate with TcZelda to regulate the development of insect wings. Additionally, Elongation (ELO), fatty acid synthase (FAS), and fatty acyl-CoA desaturase (FAD) expression was inhibited in dsZelda insects, which could disturb the lipase signaling pathways, thus, disrupting the insect reproductive system and pheromone synthesis. These results may help reveal the function of TcZelda in insects and the role of certain genes in the gene regulatory network and provide new ideas for the prevention and control of T. castaneum.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
4.00
自引率
0.00%
发文量
160
审稿时长
6-12 weeks
期刊介绍: Established in 1910, the internationally recognised Bulletin of Entomological Research aims to further global knowledge of entomology through the generalisation of research findings rather than providing more entomological exceptions. The Bulletin publishes high quality and original research papers, ''critiques'' and review articles concerning insects or other arthropods of economic importance in agriculture, forestry, stored products, biological control, medicine, animal health and natural resource management. The scope of papers addresses the biology, ecology, behaviour, physiology and systematics of individuals and populations, with a particular emphasis upon the major current and emerging pests of agriculture, horticulture and forestry, and vectors of human and animal diseases. This includes the interactions between species (plants, hosts for parasites, natural enemies and whole communities), novel methodological developments, including molecular biology, in an applied context. The Bulletin does not publish the results of pesticide testing or traditional taxonomic revisions.
期刊最新文献
Alteration in microbes changed the contents of oviposition-deterrent pheromones on the Spodoptera litura egg surface. Suppression of Cadra cautella (Lepidoptera: Pyralidae) development by phytosanitary irradiation doses and their impacts on physiochemical and microbiological quality of dates. Controlling a kisser: fermented products and commercial insects' lures as attractants of kissing bugs. Dissecting the relationships between the insect vector Empoasca paraparvipenis with Camptotheca acuminata witches' broom phytoplasma. NADPH-cytochrome P450 reductase involved in the lambda-cyhalothrin susceptibility on the green mirid bug Apolygus lucorum - Corrigendum.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1