不同来源乳酸菌中叶酸生物合成基因的发生。

IF 2.3 4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Food Technology and Biotechnology Pub Date : 2023-06-01 DOI:10.17113/ftb.61.02.23.7929
Fenny Amilia Mahara, Lilis Nuraida, Hanifah Nuryani Lioe, Siti Nurjanah
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引用次数: 0

摘要

研究背景:已知乳酸菌(LAB)可以产生叶酸。然而,这种能力是高度依赖于菌株的。特定LAB菌株的叶酸合成受叶酸有效性的影响,叶酸在一定条件下可被其他LAB消耗。此外,叶酸合成能力的差异与叶酸生物合成相关基因的存在和该途径的调控有关。实验方法:为了更好地了解不同LAB物种和菌株之间叶酸生物合成调控的基础信息,从印度尼西亚当地各种食物来源分离的产叶酸和非产叶酸LAB中筛选并鉴定了叶酸生物合成基因。采用高效液相色谱二极管阵列检测器(HPLC-DAD)分析分离菌株的细胞外叶酸产率。结果与结论:13株LAB分离株中有11株具有叶酸生物合成相关的8个基因(folE、folQ、folB、folK、folP、folC1、folA和folC2)。此外,这些分离株产生的细胞外叶酸在10.37至31.10µg/mL之间。相比之下,两个不产叶酸的分离株缺乏几个叶酸生物合成基因,如folQ、folP和folA,这可能是它们无法重新合成叶酸的原因。系统进化树分析结果显示,6个产叶酸LAB菌株的叶酸生物合成基因(不包括folK和folP)与数据库中其他LAB菌株的同源基因呈单系关系。创新与科学贡献:本研究确定了不同LAB物种中叶酸生物合成基因的分布。本研究结果支持叶酸生物合成标记基因在产叶酸LAB基因型筛选中的应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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The Occurrence of Folate Biosynthesis Genes in Lactic Acid Bacteria from Different Sources.

Research background: Lactic acid bacteria (LAB) are known to produce folate. However, this ability is highly strain-dependent. Folate synthesis in specific LAB strains is affected by the availability of folate, which can be consumed by other LAB under certain conditions. Moreover, differences in folate synthesis capabilities are related to the presence of folate biosynthesis-related genes and regulation of this pathway.

Experimental approach: As basic information to better understand the regulation of folate biosynthesis among different LAB species and strains, folate biosynthetic genes were screened and identified in folate-producing and non-folate-producing LAB isolated from various local food sources in Indonesia. The extracellular folate productivity amounts of the isolates were analyzed using high-performance liquid chromatography with a diode array detector (HPLC-DAD).

Results and conclusions: Eleven of the thirteen tested LAB isolates had all of the eight genes involved in folate biosynthesis (folE, folQ, folB, folK, folP, folC1, folA and folC2). Furthermore, these isolates produced extracellular folate ranging from 10.37 to 31.10 µg/mL. In contrast, two non-folate-producing isolates lacked several folate biosynthetic genes, such as folQ, folP and folA, which is possibly the reason for their inability to synthesize folate de novo. Phylogenetic tree construction revealed that the folate biosynthetic genes (excluding folK and folP) from six distinct species of folate-producing LAB isolates were monophyletic with homologous genes from other LAB species in the database.

Novelty and scientific contribution: In this study, the distribution of folate biosynthetic genes in various LAB species was determined. The findings from this research support the use of folate biosynthesis marker genes in the genotypic screening for folate-producing LAB.

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来源期刊
Food Technology and Biotechnology
Food Technology and Biotechnology 工程技术-生物工程与应用微生物
CiteScore
3.70
自引率
0.00%
发文量
33
审稿时长
12 months
期刊介绍: Food Technology and Biotechnology (FTB) is a diamond open access, peer-reviewed international quarterly scientific journal that publishes papers covering a wide range of topics, including molecular biology, genetic engineering, biochemistry, microbiology, biochemical engineering and biotechnological processing, food science, analysis of food ingredients and final products, food processing and technology, oenology and waste treatment. The Journal is published by the University of Zagreb, Faculty of Food Technology and Biotechnology, Croatia. It is an official journal of Croatian Society of Biotechnology and Slovenian Microbiological Society, financed by the Croatian Ministry of Science and Education, and supported by the Croatian Academy of Sciences and Arts.
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