Yelena V Grishina, Aleksey A Vatlin, Dilara A Mavletova, Maya V Odorskaya, Alexey M Senkovenko, Rustem A Ilyasov, Valeriy N Danilenko
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引用次数: 0
摘要
许多种类的乳酸菌是人类消化道的共同居住者,支持维持平衡的微生物环境,有利于宿主健康。本研究对从健康人粪便中分离出的独特乳酸菌发酵乳杆菌U-21进行了代谢谱分析,并与不具有抗氧化能力的菌株发酵乳杆菌279进行了比较。采用GC- x GC- ms鉴定各菌株代谢物指纹图谱,并对数据进行多元生物信息学分析。在体内和体外研究中,发酵乳杆菌U-21菌株已被证明具有独特的抗氧化特性,将其定位为治疗帕金森病的候选药物。代谢产物分析表明,发酵L. fermentum U-21菌株产生多种不同的化合物,证明了该菌株的独特特性。据报道,本研究中发现的一些发酵乳杆菌U-21代谢物具有促进健康的特性。基于GC × GC- ms的代谢组学试验确定菌株发酵乳杆菌U-21是一种具有显著抗氧化潜力的潜在后生物。
Metabolites Potentially Determine the High Antioxidant Properties of Limosilactobacillus fermentum U-21.
Many kinds of Lactobacillus are common occupants of humans' digestive tract that support the preservation of a balanced microbial environment that benefits host health. In this study, the unique lactic acid bacterium strain Limosilactobacillus fermentum U-21, which was isolated from the feces of a healthy human, was examined for its metabolite profile in order to compare it to that of the strain L. fermentum 279, which does not have antioxidant (AO) capabilities. By using GC × GC-MS, the metabolite fingerprint of each strain was identified, and the data were then subjected to multivariate bioinformatics analysis. The L. fermentum U-21 strain has previously been shown to possess distinctive antioxidant properties in in vivo and in vitro studies, positioning it as a drug candidate for the treatment of Parkinsonism. The production of multiple distinct compounds is shown by the metabolite analysis, demonstrating the unique characteristics of the L. fermentum U-21 strain. According to reports, some of the L. fermentum U-21 metabolites found in this study have health-promoting properties. The GC × GC-MS-based metabolomic tests defined strain L. fermentum U-21 as a potential postbiotic with significant antioxidant potential.