采用RT-PCR技术研究饲喂不同蛋白质水平对杂交地方鸡内脏重量及MEF2A和ATF3基因表达的影响。

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal, genetic engineering & biotechnology Pub Date : 2023-08-11 DOI:10.1186/s43141-023-00533-6
Alfan Kurniawan, Muhammad Halim Natsir, Suyadi Suyadi, Osfar Sjofjan, Yuli Frita Nuningtyas, Ari Ardiantoro, Ahmad Furqon, Suci Puji Lestari
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A total of 192 non-sexing crossbred local chickens day-old-chick (DOC) with a completely randomized design (CRD) method of 3 treatments and 8 replicates. Real-time polymerase chain reaction (RT-PCR) is used to measure the gene expression levels. This study aimed to determine the effect of feeding with various protein levels on internal organ weight and gene expression of MEF2A and ATF3 in crossbred local chickens.</p><p><strong>Result: </strong>The analysis of treatment revealed that the results were not significantly different (P > 0.05) on gizzard weight and spleen weight. However, it shows a significantly different result (P < 0.05) on heart weight and a highly significantly different result (P < 0.01) on pancreas weight. These findings suggest that protein levels in the diet had a significant impact on heart and pancreas weights. 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摘要

背景:Myogenic enhancer transcription factor 2A (MEF2A)是一种已知在控制骨骼肌再生和代谢过程中起作用的转录因子,而activation transcription factor 3 (ATF3)是一种应激诱导的转录因子,在调节代谢过程、免疫和肿瘤发生中起作用。环境因素,如膳食蛋白质,可以影响基因表达水平。蛋白质摄入不足会对内脏代谢性能产生负面影响,导致内脏体重异常。试验选用192只无性别杂交地方鸡(DOC),采用完全随机设计(CRD)法,分3个处理,8个重复。实时聚合酶链反应(RT-PCR)检测基因表达水平。本研究旨在研究饲喂不同蛋白质水平对杂交地方鸡内脏重量及MEF2A和ATF3基因表达的影响。结果:经治疗分析,沙囊重量和脾脏重量差异无统计学意义(P < 0.05)。结论:蛋白质水平的变化不会导致肝、肾、心、脾等脏器的体重异常。此外,MEF2A和ATF3在肌肉组织和肝脏组织中的差异基因表达模式表明,这些基因对不同蛋白质喂养处理的反应不同。这些发现提供了MEF2A和ATF3基因与杂交地方鸡蛋白质水平和器官特异性反应相关的复杂调控机制的见解。
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The effect of feeding with different protein levels on internal organ weight and gene expression of MEF2A and ATF3 in crossbred local chicken using RT-PCR.

Background: Myogenic enhancer transcription factor 2A (MEF2A) is a transcription factor known for its role in controlling skeletal muscle regeneration and metabolic processes, while activating transcription factor 3 (ATF3) is a stress-induced transcription factor that plays a role in modulating metabolic processes, immunity, and oncogenesis. Environmental factors, such as dietary protein, can influence gene expression levels. Insufficient protein intake can negatively affect the metabolic performance of internal organs, leading to the abnormal weight of internal organs. A total of 192 non-sexing crossbred local chickens day-old-chick (DOC) with a completely randomized design (CRD) method of 3 treatments and 8 replicates. Real-time polymerase chain reaction (RT-PCR) is used to measure the gene expression levels. This study aimed to determine the effect of feeding with various protein levels on internal organ weight and gene expression of MEF2A and ATF3 in crossbred local chickens.

Result: The analysis of treatment revealed that the results were not significantly different (P > 0.05) on gizzard weight and spleen weight. However, it shows a significantly different result (P < 0.05) on heart weight and a highly significantly different result (P < 0.01) on pancreas weight. These findings suggest that protein levels in the diet had a significant impact on heart and pancreas weights. In terms of gene expression, the increased utilization of protein did not result in an elevation of MEF2A gene expression in both muscle tissue and liver tissue. Specifically, in muscle tissue, MEF2A gene expression was highly expressed at 18% protein feed for the starter phase and 16% for the finisher phase. Conversely, in liver tissue, MEF2A gene expression was highly expressed at 22% protein feed for the starter phase and 20% for the finisher phase. Moreover, ATF3 gene expression in muscle tissue exhibited a negative correlation with increasing feed protein levels.

Conclusion: The results indicate that varying protein levels did not lead to abnormal weights in the liver, kidney, heart, and spleen organs. Additionally, the differential gene expression patterns of MEF2A and ATF3 in muscle tissue and liver tissue suggest that these genes respond differently to varying protein-feeding treatments. These findings provide insights into the complex regulatory mechanisms of MEF2A and ATF3 genes in relation to protein levels and organ-specific responses in crossbred local chickens.

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