Appraisal of Antioxidant Potential and Natural Bioactive Compound Determination through HPLC from Two Saprotrophic Amanita (Agaricomycetes) Species from Pakistan.

IF 1.4 4区 生物学 Q4 MYCOLOGY International journal of medicinal mushrooms Pub Date : 2023-01-01 DOI:10.1615/IntJMedMushrooms.2023048392
Muniba Shafique, Abdul Rehman Niazi, Muhammad Imran, Muhammad Zaheer
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Abstract

The present study was carried out to investigate and identify bioactive compounds along with antioxidant capacity, total flavonoids and total phenolic contents from two saprophytic Amanita species, i.e., mushrooms A. manicata (Berk. & Broome) Pegler and A. nana Singer. Antioxidant potential was assessed by DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging and ABTS (2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) assay, total phenolics, and flavonoids. Both mushrooms were found to possess antioxidants and wide range of phenolics and bioactive compounds. There was maximum percent inhibition (83.2 ± 0.120%) on DPPH by A. manicata. However, maximum percent inhibition using ABTS was found to be 79.5 ± 0.251% by A. nana. Similarly, A. nana possesses maximum amount of both flavonoids and phenolics i.e., 0.3473 ± 0.0088 mg/100 g of catechin and 0.097 ± 0.0011 mg/100 g of GAE equivalent, respectively. Both mushrooms exhibited a variety of natural compounds such as P-coumaric acid, M-coumaric acid, benzoic acid, ferulic acid etc. Ferulic acid, chlorogenic acid, and cinnamic acid were also detected in A. manicata. A. manicata exhibited best and greater antiradical potential than A. nana due the presence of excessive natural bioactive compounds. From the reported results, it was revealed that both inedible mushrooms could be a potential source of antioxidants and secondary metabolites and might be used for making novel drugs in future by pharmaceutical industries.

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巴基斯坦两种腐养型毒伞菌抗氧化活性评价及天然生物活性成分的HPLC测定。
本研究对腐生毒伞属蘑菇A. manicata (Berk.)的活性成分、抗氧化能力、总黄酮和总酚含量进行了研究和鉴定。&布鲁姆)佩格勒和A.娜娜辛格。通过DPPH(2,2-二苯基-1-吡啶肼基)自由基清除能力和ABTS(2,2'-氮基-双-3-乙基苯并噻唑-6-磺酸)测定、总酚类物质和类黄酮测定来评估其抗氧化能力。这两种蘑菇都含有抗氧化剂和广泛的酚类物质和生物活性化合物。马齿苋对DPPH的抑制率最高(83.2±0.120%)。ABTS的最大抑菌率为79.5±0.251%。同样,黄酮类化合物和酚类物质含量最高,分别为0.3473±0.0088 mg/100 g儿茶素和0.097±0.0011 mg/100 g GAE当量。两种蘑菇均含有对香豆酸、间香豆酸、苯甲酸、阿魏酸等多种天然化合物。马甲中还检出阿魏酸、绿原酸和肉桂酸。由于含有较多的天然活性物质,马齿苋表现出较强的抗自由基能力。从报道的结果来看,这两种不可食用蘑菇都可能是抗氧化剂和次生代谢物的潜在来源,并可能在未来被制药工业用于制造新药。
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来源期刊
CiteScore
2.60
自引率
16.70%
发文量
91
审稿时长
6-12 weeks
期刊介绍: The rapid growth of interest in medicinal mushrooms research is matched by the large number of disparate groups that currently publish in a wide range of publications. The International Journal of Medicinal Mushrooms is the one source of information that will draw together all aspects of this exciting and expanding field - a source that will keep you up to date with the latest issues and practice. The International Journal of Medicinal Mushrooms published original research articles and critical reviews on a broad range of subjects pertaining to medicinal mushrooms, including systematics, nomenclature, taxonomy, morphology, medicinal value, biotechnology, and much more.
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