Chemical Characterization and Biological Functions of Hot Alkali-Soluble Crude Polysaccharide from the Water-Insoluble Residue of Macrocybe lobayensis (Agaricomycetes) Fruit Bodies.

Abstract

Macrocybe lobayensis owe popularity in several traditional cultures not only for delectable taste but also for its nutraceutical profile conveying great health benefits. Previous investigations have enumerated several bioactivities of the valuable mushroom such as antioxidant, anti-ageing, immune-modulation, and anti-tumor properties where polysaccharides played the key role. Macrofungi polymers are generally isolated by the conventional hot water process discarding the residue which still contains plenty of therapeutic components. The present study thus aimed to re-use such leftover of the edible macrofungus by immersing it in NaOH solution at high temperature supporting circular economy. Consequently a polysaccharide fraction, namely ML-HAP, was isolated that was found to be consisted of a homogenous heteropolysaccharide with molecular weight of ~ 128 kDa and β-glucan as the chief ingredient as evident by spectroscopy, gel-permeation chromatography, high performance thin-layer chromatography, and Fourier transform infrared. Antioxidant activity assays revealed that the macromolecules possess good radical scavenging, metal ion binding and reducing power. Nevertheless, strong immune-potentiation was also recorded as the extract triggered murine macrophage cell viability, pinocytosis, nitric oxide production and morpho-dynamics within 24 h where the best effect was executed at the level of 100 µg/mL. Altogether, the polysaccharides extracted from M. lobayensis exhibited a potent application prospect in functional food, pharmaceutical, nutraceutical and health care industries that could raise economic value of the underexplored mushroom.