Frequent detection and genotyping of human rhinovirus in SARS-CoV-2 negative patients; a study from south of Iran.

IF 1.3 Q4 MICROBIOLOGY Iranian Journal of Microbiology Pub Date : 2023-06-01 DOI:10.18502/ijm.v15i3.12908

Amirhossein Darabi, Behrang Sarshari, Katayoun Vahdat, Seyed Alireza Mousavi, Mohsen Keshavarz

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Abstract

Background and objectives: Human rhinovirus (HRV), a major cause of common cold, was associated to the hospitalization of children and adults. This cross-sectional study aimed to determine the prevalence, and genotype distribution of HRV in the patients with mild to severe respiratory infections who were negative for SARS-Cov-2.

Materials and methods: Nasopharyngeal swab specimens (n = 356) from the patients aged 29 days to 82 years, received for the respiratory virus detection from January to December 2021, were analyzed for human rhinovirus (HRV) by RT-PCR. As a final step, genotyping was performed on obtained sequences.

Results: A total of 37 HRV infections were identified (37/356, 10%). The highest rates of positive HRV tests were observed in February (21.6%), and January (18.9%), compared with June and August (0%). HRV-positive cases mainly appeared in winter. Among the age groups, those 2-<5 years of age had the highest detection rate (21%), however, those >55 years of age had the lowest detection rate (3%). Among HRV-positive samples, 30 (81%) were identified as type HRV-A, 5 (13.5%) as HRV-B, and 2 (5.5%) as HRV-C.

Conclusion: Our results suggested that HRV frequency gradually decreased with the age of patients which is more active in Iran, especially in the cold months.

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SARS-CoV-2阴性患者鼻病毒频繁检测及基因分型一项来自伊朗南部的研究。

背景和目的:人鼻病毒(HRV)是引起普通感冒的主要原因，与儿童和成人住院有关。本横断面研究旨在确定在SARS-Cov-2阴性的轻至重度呼吸道感染患者中HRV的患病率和基因型分布。材料与方法:采用RT-PCR方法对2021年1 - 12月用于呼吸道病毒检测的29天~ 82岁患者鼻咽拭子标本356份进行人鼻病毒(HRV)检测。作为最后一步，对获得的序列进行基因分型。结果:共检出HRV感染37例(37/ 356,10 %)。与6月和8月(0%)相比，2月(21.6%)和1月(18.9%)HRV检测阳性率最高。hrv阳性病例主要出现在冬季。各年龄组中，2 ~ 55岁检出率最低(3%)。在hrv阳性样本中，HRV-A型30例(81%)，HRV-B型5例(13.5%)，HRV-C型2例(5.5%)。结论:随着患者年龄的增长，HRV频率逐渐降低，在伊朗尤其在寒冷月份更为活跃。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.