Male vitellogenin regulates gametogenesis through a testis-enriched big protein in Chrysopa pallens

IF 2.3 2区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Insect Molecular Biology Pub Date : 2023-09-14 DOI:10.1111/imb.12873
Xiao-ping Liu, Chang-yan Liu, Yan-jiao Feng, Xing-kai Guo, Li-sheng Zhang, Meng-qing Wang, Yu-yan Li, Fan-rong Zeng, Tony Nolan, Jian-jun Mao
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Abstract

In insects, vitellogenin (Vg) is generally viewed as a female-specific protein. Its primary function is to supply nutrition to developing embryos. Here, we reported Vg from the male adults of a natural predator, Chrysopa pallens. The male Vg was depleted by RNAi. Mating with Vg-deficient male downregulated female Vg expression, suppressed ovarian development and decreased reproductive output. Whole-organism transcriptome analysis after male Vg knockdown showed no differential expression of the known spermatogenesis-related regulators and seminal fluid protein genes, but a sharp downregulation of an unknown gene, which encodes a testis-enriched big protein (Vcsoo). Separate knockdown of male Vg and Vcsoo disturbed the assembly of spermatid cytoplasmic organelles in males and suppressed the expansion of ovary germarium in mated females. These results demonstrated that C. pallens male Vg signals through the downstream Vcsoo and regulates male and female reproduction.

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雄性卵黄蛋白原通过一种富含睾丸的大蛋白来调节淡色金藻的配子发生。
在昆虫中,卵黄蛋白原(Vg)通常被认为是一种雌性特有的蛋白质。它的主要功能是为发育中的胚胎提供营养。在这里,我们报道了来自一种天然食肉动物——帕勒斯金藻雄性成虫的Vg。雄性Vg被RNAi耗尽。与缺乏Vg的雄性交配可下调雌性Vg的表达,抑制卵巢发育并降低生殖产量。雄性Vg敲低后的全生物转录组分析显示,已知的精子发生相关调节因子和精液蛋白基因没有差异表达,但一个未知基因急剧下调,该基因编码一种富含睾丸的大蛋白(Vcsoo)。雄性Vg和Vcsoo的单独敲除干扰了雄性精子细胞器的组装,并抑制了交配雌性卵巢生殖库的扩张。这些结果表明,C.pallens雄性Vg通过下游Vcsoo发出信号,并调节雄性和雌性繁殖。
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来源期刊
Insect Molecular Biology
Insect Molecular Biology 生物-昆虫学
CiteScore
4.80
自引率
3.80%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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