CAMSAP2 and CAMSAP3 localize at microtubule intersections to regulate the spatial distribution of microtubules.

IF 5.3 2区 生物学 Q2 CELL BIOLOGY Journal of Molecular Cell Biology Pub Date : 2024-01-17 DOI:10.1093/jmcb/mjad050
Rui Zhang, Lusheng Gu, Wei Chen, Nobutoshi Tanaka, Zhengrong Zhou, Honglin Xu, Tao Xu, Wei Ji, Xin Liang, Wenxiang Meng
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Abstract

Microtubule networks support many cellular processes and exhibit a highly ordered architecture. However, due to the limited axial resolution of conventional light microscopy, the structural features of these networks cannot be resolved in three-dimensional (3D) space. Here, we used customized ultra-high-resolution interferometric single-molecule localization microscopy to characterize the microtubule networks in Caco2 cells. We found that the calmodulin-regulated spectrin-associated proteins (CAMSAPs) localize at a portion of microtubule intersections. Further investigation showed that depletion of CAMSAP2 and CAMSAP3 leads to the narrowing of the inter-microtubule distance. Mechanistically, CAMSAPs recognize microtubule defects, which often occur near microtubule intersections, and then recruit katanin to remove the damaged microtubules. Therefore, the CAMSAP-katanin complex is a regulatory module for the distance between microtubules. Taken together, our results characterize the architecture of cellular microtubule networks in high resolution and provide molecular insights into how the 3D structure of microtubule networks is controlled.

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CAMSAP2 和 CAMSAP3 定位于微管交汇处,调节微管的空间分布。
微管网络支持许多细胞过程,并表现出高度有序的结构。然而,由于传统光学显微镜的轴向分辨率有限,这些网络的结构特征无法在三维(3D)空间中解析。在这里,我们使用定制的超高分辨率干涉单分子定位显微镜来表征 Caco2 细胞中的微管网络。我们发现,钙调蛋白调控的光谱蛋白相关蛋白(CAMSAPs)定位在部分微管交汇处。进一步的研究表明,消耗 CAMSAP2 和 CAMSAP3 会导致微管间距离变窄。从机理上讲,CAMSAPs 可识别微管缺陷(通常发生在微管交叉点附近),然后招募 katanin 移除受损的微管。因此,CAMSAP-katanin 复合物是微管间距的调控模块。综上所述,我们的研究结果以高分辨率描述了细胞微管网络的结构,并提供了关于如何控制微管网络三维结构的分子见解。
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来源期刊
CiteScore
9.60
自引率
1.80%
发文量
1383
期刊介绍: The Journal of Molecular Cell Biology ( JMCB ) is a full open access, peer-reviewed online journal interested in inter-disciplinary studies at the cross-sections between molecular and cell biology as well as other disciplines of life sciences. The broad scope of JMCB reflects the merging of these life science disciplines such as stem cell research, signaling, genetics, epigenetics, genomics, development, immunology, cancer biology, molecular pathogenesis, neuroscience, and systems biology. The journal will publish primary research papers with findings of unusual significance and broad scientific interest. Review articles, letters and commentary on timely issues are also welcome. JMCB features an outstanding Editorial Board, which will serve as scientific advisors to the journal and provide strategic guidance for the development of the journal. By selecting only the best papers for publication, JMCB will provide a first rate publishing forum for scientists all over the world.
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