Can PCNA and LIM15 gene expression levels predict sperm retrieval success in men with non-obstructive azoospermia?

Abstract

Introduction and objectives

It is necessary to be able to predict sperm retrieval before microdissection testicular sperm extraction (mTESE) in azoospermic men. This study established the importance of proliferating cell nuclear antigen (PCNA) and LIM15 gene expression levels in predicting the success of sperm retrieval by mTESE.

Materials and methods

One hundred and forty-three men who were diagnosed with non-obstructive azoospermia (NOA) were included in the study. Patients’ age, total testosterone and follicle stimulating hormone values, testicular volume and testicular histology were recorded by prospectively. PCNA and LIM15 gene expression levels were determined by real-time PCR in the materials from both ejaculate and testicular specimens.

Results

Testis volume and histology were the most important factors in predicting the sperm retrieval rate (SRR). The PCNA and LIM15 gene expression levels measured in testicular tissues and the LIM15 gene expression levels measured in ejaculate significantly correlated with the SRR in mTESE (p = 0.038, p = 0.022, and p = 0.004, respectively). Although the PCNA gene expression level measured in ejaculate was higher in men with successful sperm retrieval, the difference was not statistically significant (p = 0.061). According to the multivariate logistic regression analysis, testicular volume and LIM15 gene expression level in ejaculate were independent predictive parameters for sperm retrieval.

Conclusion

The data showed that LIM15 gene expression level in ejaculate is a useful molecular marker to predict the SRR before mTESE.