Biosynthesis of Zinc Oxide Nanoparticles by Lactobacillus spp. and Investigation of their Antimicrobial Effect.

Q3 Pharmacology, Toxicology and Pharmaceutics Current drug discovery technologies Pub Date : 2023-01-01 DOI:10.2174/1570163820666230501152951

Saeideh Morovaty Sharifabady, Pegah Shakib, Behin Omidi, Mohammad Reza Zolfaghari

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Abstract

Background: Nanoparticle biology is preferable to other common methods due to its economic efficiency and compatibility with the environment. On the other hand, the prevalence of drug-resistant bacterial strains is expanding and it is necessary to use alternative antibiotic compounds to deal with them. The aim of the present study was the biosynthesis of zinc oxide nanoparticles(ZnO NPs) by Lactobacillus spp. and their antimicrobial effect.

Methods: In this study, after the biosynthesis of ZnO NPs by Lactobacillus spp, Characterization of Nanoparticulation Was performed by UV-Vis, XRD, and Scanning Electron Microscopy (SEM). Additionally, Lactobacillus spp. - ZnO NPs were assessed for their antimicrobial properties.

Results: UV-visible spectroscopy confirmed the Lactobacillus spp. - ZnO NPs absorbed UV in the region of 300-400 nm. XRD analysis showed the presence of zinc metal in nanoparticles. SEM revealed that Lactobacillus plantarum - ZnO NPs were smaller than the others. Staphylococcus aureus showed the largest non-growth halo diameter against ZnO NPs synthesized by L. plantarum ATCC 8014 (3.7 mm). E. coli had the largest growth halo diameter against ZnO NPs synthesized by L. casei (3 mm) and L. plantarum (2.9 mm). The MIC values of ZnO NPs synthesized by L. plantarum ATCC 8014, L.casei ATCC 39392, L. fermenyum ATCC 9338, L. acidophilus ATCC 4356 were 2,8,8 and 4 μg/mL for Staphylococcus aureus. The MIC values of ZnO NPs synthesized by L. plantarum ATCC 8014, L. casei ATCC 39392, L. fermenyum ATCC 9338, L. acidophilus ATCC 4356 were 2, 4, 4, and 4 μg/ml for E. coli. The lowest MICs were 2 μg/ml for E. coli and S. aureus related to ZnO NPs synthesized by L. plantarum ATCC 8014. MIC and MBC values were equivalent to each other.

Conclusion: The results of this research show that ZnO NPs synthesized by L. plantarum ATCC 8014 have more antimicrobial effects than other ZnO NPs used. Therefore, the ZnO NPs made with Lactobacillus plantarum ATCC 8014 have the potential to kill bacteria and can be considered a candidate for antibiotic replacement.

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乳杆菌合成氧化锌纳米颗粒及其抑菌效果研究。

背景:纳米颗粒生物学由于其经济效率和与环境的相容性而优于其他常用方法。另一方面，耐药菌株的流行正在扩大，有必要使用替代抗生素化合物来处理它们。研究了乳杆菌合成氧化锌纳米颗粒及其抑菌效果。方法:本研究通过乳酸杆菌合成氧化锌NPs后，利用UV-Vis、XRD和扫描电镜(SEM)对纳米连接进行表征。此外，还对乳酸菌- ZnO NPs的抗菌性能进行了评价。结果:通过紫外可见光谱分析，证实乳酸菌- ZnO NPs在300 ~ 400 nm范围内吸收紫外。XRD分析表明，纳米颗粒中存在金属锌。扫描电镜显示，植物乳杆菌氧化锌NPs比其他菌株的NPs小。金黄色葡萄球菌对L. plantarum ATCC 8014合成的ZnO NPs的非生长晕直径最大(3.7 mm)。大肠杆菌对干酪乳杆菌(L. casei)和植物乳杆菌(L. plantarum)合成的ZnO NPs的生长晕直径最大(3 mm)。植物乳杆菌ATCC 8014、干酪乳杆菌ATCC 39392、发酵乳杆菌ATCC 9338、嗜酸乳杆菌ATCC 4356合成的氧化锌NPs对金黄色葡萄球菌的MIC值分别为2、8、8和4 μg/mL。植物乳杆菌ATCC 8014、干酪乳杆菌ATCC 39392、发酵乳杆菌ATCC 9338、嗜酸乳杆菌ATCC 4356合成的氧化锌NPs对大肠杆菌的MIC值分别为2、4、4和4 μg/ml。与植物乳杆菌ATCC 8014合成的氧化锌NPs相关的大肠杆菌和金黄色葡萄球菌mic最低均为2 μg/ml。MIC值与MBC值相等。结论:利用L. plantarum ATCC 8014合成的ZnO NPs具有较好的抑菌效果。因此，用植物乳杆菌ATCC 8014制备的ZnO NPs具有杀灭细菌的潜力，可以被认为是抗生素替代的候选物。

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Current drug discovery technologies Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

3.70

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0.00%

发文量

期刊介绍： Due to the plethora of new approaches being used in modern drug discovery by the pharmaceutical industry, Current Drug Discovery Technologies has been established to provide comprehensive overviews of all the major modern techniques and technologies used in drug design and discovery. The journal is the forum for publishing both original research papers and reviews describing novel approaches and cutting edge technologies used in all stages of drug discovery. The journal addresses the multidimensional challenges of drug discovery science including integration issues of the drug discovery process.