Recombinant duck enteritis virus bearing the hemagglutinin genes of H5 and H7 influenza viruses is an ideal multivalent live vaccine in ducks.

IF 8.4 2区医学 Q1 IMMUNOLOGY Emerging Microbes & Infections Pub Date : 2024-12-01 Epub Date: 2024-01-04 DOI:10.1080/22221751.2023.2284301

Yubo Zhao, Pucheng Chen, Yuzhen Hu, Jing Liu, Yongping Jiang, Xianying Zeng, Guohua Deng, Jianzhong Shi, Yanbing Li, Guobin Tian, Jinxiong Liu, Hualan Chen

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Abstract

Due to the fact that many avian influenza viruses that kill chickens are not lethal to ducks, farmers are reluctant to use avian influenza inactivated vaccines on ducks. Large numbers of unvaccinated ducks play an important role in the transmission of avian influenza viruses from wild birds to domestic poultry, creating a substantial challenge to vaccination strategies for avian influenza control. To solve this problem, we constructed a recombinant duck enteritis virus (DEV), rDEV-dH5/H7, using a live attenuated DEV vaccine strain (vDEV) as a vector. rDEV-dH5/H7 carries the hemagglutinin gene of two H5 viruses [GZ/S4184/17 (H5N6) (clade 2.3.4.4 h) and LN/SD007/17 (H5N1) (clade 2.3.2.1d)] and an H7 virus [GX/SD098/17 (H7N9)]. These three hemagglutinin genes were stably inherited in rDEV-dH5/H7 and expressed in rDEV-dH5/H7-infected cells. Animal studies revealed that rDEV-dH5/H7 and vDEV induced similar neutralizing antibody responses and protection against lethal DEV challenge. Importantly, rDEV-dH5/H7 induced strong and long-lasting hemagglutinin inhibition antibodies against different H5 and H7 viruses and provided complete protection against challenges with homologous and heterologous highly pathogenic H5 and H7 influenza viruses in ducks. Our study shows that rDEV-dH5/H7 could serve as an ideal live attenuated vaccine to protect ducks against infection with lethal DEV and highly pathogenic avian influenza viruses.

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携带H5和H7流感病毒血凝素基因的重组鸭肠炎病毒是一种理想的鸭多价活疫苗。

由于许多杀死鸡的禽流感病毒对鸭并不致命，因此农民不愿意在鸭身上使用禽流感灭活疫苗。大量未接种疫苗的鸭子在禽流感病毒从野鸟向家禽传播方面发挥了重要作用，对禽流感控制的疫苗接种战略构成了重大挑战。为了解决这一问题，我们以鸭肠炎病毒(DEV)减毒活疫苗株(vDEV)为载体，构建了重组鸭肠炎病毒(DEV) rDEV-dH5/H7。rDEV-dH5/H7携带两种H5病毒[GZ/S4184/17 (H5N6)(进化枝2.3.4.4 h)和LN/SD007/17 (H5N1)(进化枝2.3.2.1d)]和一种H7病毒[GX/SD098/17 (H7N9)]的血凝素基因。这三个血凝素基因在rDEV-dH5/H7中稳定遗传，并在rDEV-dH5/H7感染的细胞中表达。动物实验表明，rDEV-dH5/H7和vDEV诱导了相似的中和抗体反应和对致命DEV攻击的保护作用。重要的是，rDEV-dH5/H7诱导了针对不同H5和H7病毒的强效和持久的血凝素抑制抗体，并对鸭同源和异源高致病性H5和H7流感病毒的攻击提供了完全的保护。本研究表明，rDEV-dH5/H7可作为一种理想的减毒活疫苗，保护鸭子免受致命的DEV和高致病性禽流感病毒的感染。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Emerging Microbes & Infections IMMUNOLOGY-MICROBIOLOGY

CiteScore

26.20

自引率

2.30%

发文量

276

审稿时长

20 weeks

期刊介绍： Emerging Microbes & Infections is a peer-reviewed, open-access journal dedicated to publishing research at the intersection of emerging immunology and microbiology viruses. The journal's mission is to share information on microbes and infections, particularly those gaining significance in both biological and clinical realms due to increased pathogenic frequency. Emerging Microbes & Infections is committed to bridging the scientific gap between developed and developing countries. This journal addresses topics of critical biological and clinical importance, including but not limited to: - Epidemic surveillance - Clinical manifestations - Diagnosis and management - Cellular and molecular pathogenesis - Innate and acquired immune responses between emerging microbes and their hosts - Drug discovery - Vaccine development research Emerging Microbes & Infections invites submissions of original research articles, review articles, letters, and commentaries, fostering a platform for the dissemination of impactful research in the field.