Comparison of Characterization in Two-Dimensional and Three-Dimensional Canine Mammary Gland Tumor Cell Models.

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Yonago acta medica Pub Date : 2023-02-01 DOI:10.33160/yam.2023.02.002

Tomohiro Osaki, Yuji Sunden, Katsuhiko Warita, Yoshiharu Okamoto

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Abstract

Background: Canine mammary gland tumors can be used as predictive models for human breast cancer. There are several types of microRNAs common in human breast cancer and canine mammary gland tumors. The functions of microRNAs in canine mammary gland tumors are not well understood.

Methods: We compared the characterization of microRNA expression in two-dimensional and three-dimensional canine mammary gland tumor cell models. We evaluated the differences between two- and three-dimensional cultured canine mammary gland tumor SNP cells by assessing microRNA expression levels, morphology, drug sensitivity, and hypoxia.

Results: The expression of microRNA-210 in the three-dimensional-SNP cells was 10.19 times higher than that in the two-dimensional-SNP cells. The intracellular concentrations of doxorubicin in the two- and three-dimensional-SNP cells were 0.330 ± 0.013 and 0.290 ± 0.048 nM/mg protein, respectively. The IC₅₀ values of doxorubicin for the two- and three-dimensional-SNP cells were 5.2 and 1.6 μM, respectively. Fluorescence of the hypoxia probe, LOX-1, was observed inside the sphere of three-dimensional-SNP cells without echinomycin but not in two-dimensional-SNP cells. The three-dimensional-SNP cells treated with echinomycin showed weak LOX-1 fluorescence.

Conclusion: The present study showed a clear difference in microRNA expression levels in cells cultured in a two-dimensional adherent versus a three-dimensional spheroid model.

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二维与三维犬乳腺肿瘤细胞模型表征的比较。

背景:犬乳腺肿瘤可作为人类乳腺癌的预测模型。在人类乳腺癌和犬乳腺肿瘤中有几种常见的microrna。microrna在犬乳腺肿瘤中的作用尚不清楚。方法:比较二维和三维犬乳腺肿瘤细胞模型中microRNA的表达特征。我们通过评估microRNA表达水平、形态、药物敏感性和缺氧来评估二维和三维培养的犬乳腺肿瘤SNP细胞之间的差异。结果:microRNA-210在三维snp细胞中的表达量是二维snp细胞的10.19倍。二维和三维snp细胞内阿霉素浓度分别为0.330±0.013和0.290±0.048 nM/mg蛋白。阿霉素对二维和三维snp细胞的IC50值分别为5.2 μM和1.6 μM。缺氧探针LOX-1的荧光在不含棘霉素的三维snp细胞的球内观察到，而在二维snp细胞中未观察到。棘霉素处理的三维snp细胞显示弱的LOX-1荧光。结论:本研究显示，在二维贴壁和三维球体模型中培养的细胞中，microRNA的表达水平有明显差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Yonago acta medica MEDICINE, RESEARCH & EXPERIMENTAL-

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Yonago Acta Medica (YAM) is an electronic journal specializing in medical sciences, published by Tottori University Medical Press, 86 Nishi-cho, Yonago 683-8503, Japan. The subject areas cover the following: molecular/cell biology; biochemistry; basic medicine; clinical medicine; veterinary medicine; clinical nutrition and food sciences; medical engineering; nursing sciences; laboratory medicine; clinical psychology; medical education. Basically, contributors are limited to members of Tottori University and Tottori University Hospital. Researchers outside the above-mentioned university community may also submit papers on the recommendation of a professor, an associate professor, or a junior associate professor at this university community. Articles are classified into four categories: review articles, original articles, patient reports, and short communications.