A quantitative analysis of macrophage-colony-stimulating factor in peri-miniscrew implant crevicular fluid before and after orthodontic loading.

IF 3 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Angle Orthodontist Pub Date : 2022-11-11 DOI:10.2319/062322-445.1

Sakshi Katyal, Om Prakash Kharbanda, Ritu Duggal, Vilas D Samrit

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Abstract

Objectives: To analyze macrophage-colony-stimulating factor (M-CSF), a bone remodeling biomarker in the peri-miniscrew implant crevicular fluid (PMICF) after insertion and orthodontic loading.

Materials and methods: This prospective study included 40 miniscrew implant (MSI) sites in 10 subjects undergoing fixed orthodontic mechanotherapy utilizing miniscrew anchorage. After dental alignment, miniscrews were inserted between the second premolar and first molar roots. After 21 days of insertion, MSIs were direct loaded with closed-coil springs (200 g force) for en masse retraction of anterior teeth. PMICF was collected with Periopaper™ strips from the gingival crevice around MSIs at six time points (T1-T6: 1 hour, 1 day, 21 days postinsertion, and 7, 21, and 42 days postloading). PMICF was quantified for M-CSF by enzyme-linked immunosorbent assay. Paired comparison of mean M-CSF concentrations before and after loading stages (T1-T6) was made using the Wilcoxon signed-rank test.

Results: The mean M-CSF concentration showed a significant peak at T3 (21 days postinsertion; 12.646 pg/mL; T1 vs T3: P < .0001). After orthodontic loading of miniscrews, M-CSF levels increased to 13.570 pg/mL at T4 (7 days after loading; T1 vs T4: P < .001) and maintained at a plateau to T5 (21 days postloading; 11.994 pg/mL). However, the difference between preloading and postloading was not statistically significant (T3 vs T4).

Conclusions: The maximum M-CSF activity around MSIs was observed at around 3 weeks of miniscrew insertion, suggesting underlying bone remodeling after surgical injury. However, orthodontic force on MSIs did not cause any significant surge in M-CSF levels postloading.

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正畸前后种植体周围缝隙液中巨噬细胞集落刺激因子的定量分析

研究目的分析植入和正畸加载后迷你螺钉种植体周围缝隙液（PMICF）中的骨重塑生物标志物--巨噬细胞-菌落刺激因子（M-CSF）：这项前瞻性研究包括10名接受固定正畸机械疗法的受试者的40个微型螺钉种植体（MSI）部位，使用微型螺钉固定。对齐牙齿后，在第二前磨牙和第一磨牙根之间植入微型螺钉。插入 21 天后，MSI 直接装入闭合线圈弹簧（200 克力），用于前牙的整体牵引。在六个时间点（T1-T6：插入后 1 小时、1 天、21 天，以及加载后 7 天、21 天和 42 天）用 Periopaper™ 纸条从 MSIs 周围的龈沟收集 PMICF。通过酶联免疫吸附试验对 PMICF 的 M-CSF 进行量化。使用 Wilcoxon 符号秩检验对加载阶段（T1-T6）前后的 M-CSF 平均浓度进行配对比较：结果：M-CSF的平均浓度在T3阶段出现了明显的峰值（植入后21天；12.646 pg/mL；T1 vs T3：P < .0001）。微型螺钉正畸加载后，M-CSF 水平在 T4（加载后 7 天；T1 vs T4：P < .001）时增至 13.570 pg/mL，并在 T5（加载后 21 天；11.994 pg/mL）时维持在高位。然而，加载前和加载后的差异没有统计学意义（T3 vs T4）：结论：MSI周围的M-CSF活性在微型螺钉插入后3周左右达到最高，表明手术损伤后潜在的骨重塑。然而，对 MSIs 施加正畸力并不会导致加载后的 M-CSF 水平显著上升。

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来源期刊

Angle Orthodontist 医学-牙科与口腔外科

CiteScore

6.40

自引率

5.90%

发文量

审稿时长

3 months

期刊介绍： The Angle Orthodontist is the official publication of the Edward H. Angle Society of Orthodontists and is published bimonthly in January, March, May, July, September and November by The EH Angle Education and Research Foundation Inc. The Angle Orthodontist is the only major journal in orthodontics with a non-commercial, non-profit publisher -- The E. H. Angle Education and Research Foundation. We value our freedom to operate exclusively in the best interests of our readers and authors. Our website www.angle.org is completely free and open to all visitors.