Current methods to analyze lysosome morphology, positioning, motility and function.

IF 3.6 3区生物学 Q3 CELL BIOLOGY Traffic Pub Date : 2022-05-01 Epub Date: 2022-04-24 DOI:10.1111/tra.12839

Duarte C Barral, Leopoldo Staiano, Cláudia Guimas Almeida, Dan F Cutler, Emily R Eden, Clare E Futter, Antony Galione, André R A Marques, Diego Luis Medina, Gennaro Napolitano, Carmine Settembre, Otília V Vieira, Johannes M F G Aerts, Peace Atakpa-Adaji, Gemma Bruno, Antonella Capuozzo, Elvira De Leonibus, Chiara Di Malta, Cristina Escrevente, Alessandra Esposito, Paolo Grumati, Michael J Hall, Rita O Teodoro, Susana S Lopes, J Paul Luzio, Jlenia Monfregola, Sandro Montefusco, Frances M Platt, Roman Polishchuck, Maria De Risi, Irene Sambri, Chiara Soldati, Miguel C Seabra

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Abstract

Since the discovery of lysosomes more than 70 years ago, much has been learned about the functions of these organelles. Lysosomes were regarded as exclusively degradative organelles, but more recent research has shown that they play essential roles in several other cellular functions, such as nutrient sensing, intracellular signalling and metabolism. Methodological advances played a key part in generating our current knowledge about the biology of this multifaceted organelle. In this review, we cover current methods used to analyze lysosome morphology, positioning, motility and function. We highlight the principles behind these methods, the methodological strategies and their advantages and limitations. To extract accurate information and avoid misinterpretations, we discuss the best strategies to identify lysosomes and assess their characteristics and functions. With this review, we aim to stimulate an increase in the quantity and quality of research on lysosomes and further ground-breaking discoveries on an organelle that continues to surprise and excite cell biologists.

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目前分析溶酶体形态、定位、运动和功能的方法。

自从 70 多年前发现溶酶体以来，人们对这些细胞器的功能有了很多了解。溶酶体曾被认为是唯一的降解细胞器，但最近的研究表明，溶酶体在营养传感、细胞内信号传导和新陈代谢等其他几种细胞功能中发挥着重要作用。方法学的进步对我们目前了解这种多层面细胞器的生物学特性起到了关键作用。在这篇综述中，我们将介绍目前用于分析溶酶体形态、定位、运动和功能的方法。我们将重点介绍这些方法背后的原理、方法策略及其优势和局限性。为了提取准确的信息并避免误读，我们讨论了识别溶酶体并评估其特征和功能的最佳策略。通过这篇综述，我们希望促进溶酶体研究数量和质量的提高，并进一步推动细胞生物学家对这一细胞器的突破性发现。

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来源期刊

Traffic 生物-细胞生物学

CiteScore

8.10

自引率

2.20%

发文量

审稿时长

2 months

期刊介绍： Traffic encourages and facilitates the publication of papers in any field relating to intracellular transport in health and disease. Traffic papers span disciplines such as developmental biology, neuroscience, innate and adaptive immunity, epithelial cell biology, intracellular pathogens and host-pathogen interactions, among others using any eukaryotic model system. Areas of particular interest include protein, nucleic acid and lipid traffic, molecular motors, intracellular pathogens, intracellular proteolysis, nuclear import and export, cytokinesis and the cell cycle, the interface between signaling and trafficking or localization, protein translocation, the cell biology of adaptive an innate immunity, organelle biogenesis, metabolism, cell polarity and organization, and organelle movement. All aspects of the structural, molecular biology, biochemistry, genetics, morphology, intracellular signaling and relationship to hereditary or infectious diseases will be covered. Manuscripts must provide a clear conceptual or mechanistic advance. The editors will reject papers that require major changes, including addition of significant experimental data or other significant revision. Traffic will consider manuscripts of any length, but encourages authors to limit their papers to 16 typeset pages or less.