The effect Escherichia coli lipopolysaccharide (endotoxin) on the graft-versus-host reaction in mice (IV).

Abstract

Lipopolysaccharide from Escherichia coli (LPS) administered to mice during the graft-versus-host (GvH) reaction in a single dose of 100 mug stimulated the reaction if it was given on the same day as the parental spleen cells, or inhibited it if given four days before injection of the cells. The GvH reaction was intensified when LPS was injected in the dose of 20 mug on the fourth day after injection of the cells. The parental spleen cells responsible for the intestifying effect on GvH were sensitive to hydrocortisone, nonadherent, and probably develop in the course of the first week outside the donor spleen and settle in the spleen in a later stage of the GvH reaction.