Electrophoretic studies on nuclear RNA extracted from the spleens of immunized mice are reported. RNA was extracted by the phenol-detergent procedure from the purified nuclei of spleen cells at the peak of primary immune response after a single immunization with sheep red blood cells. The kinetics of labeling in vivo with 32P-orthophosphate indicate an accelerated biosynthesis of the ribosomal RNA precursors, 28S, 18S, 5S and 4S fractions of nuclear RNA in the spleens of immunized mice.
Lipid mobilizing activity is lowered in the serum of obese patients. During short-term fasting, this activity increases in non-obese subjects. The present study was concerned with the changes in lipid mobilizing activity in the blood of obese patients during prolonged fasting, and with the behavior of esterified and free fatty acids. Serum levels of free fatty acids in the control subjects were significantly lower than in the obese women. During a 14-day period of complete fasting, levels of free fatty acids increased in all cases. In non-obese subjects this was accompanied by a significant rise in lipid mobilizing acitivity, but in obese subjects no significant change took place in this activity. The findings indicate that high levels of free fatty acids in obese persons during fasting are caused by inhibition of their esterification.
Lipolytic activity of human placenta and lipid mobilizing activity (LMA), free fatty acids (FFA) as well as esterified fatty acid (EFA) levels in serum of pregnant women in different periods of pregnancy and in newborn's serum were studied. Lipolytic activity of human placenta is low in the first trimester of pregnancy and increases in samples examined after delivery. The levels of FFA and EFA in blood of pregnant women are significantly increased in the second and third trimester of pregnancy. The newborn's blood lipid level is significantly lower than that in the mother's blood. Lipid mobilizing activity in serum of pregnant women increases especially in the third trimester of pregnancy and is also high in newborn's blood. This may indicate that fatty acids released from newborn's adipose tissue are metabolized as a source of energy.
Human lymphocytes cultured for 3-8 days in the presence of autologous or allogenic spermatozoa undergo blast transformation. Possible mechanisms of the response are discussed.
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