Electrophoretic studies on nuclear RNA extracted from the spleens of immunized mice are reported. RNA was extracted by the phenol-detergent procedure from the purified nuclei of spleen cells at the peak of primary immune response after a single immunization with sheep red blood cells. The kinetics of labeling in vivo with 32P-orthophosphate indicate an accelerated biosynthesis of the ribosomal RNA precursors, 28S, 18S, 5S and 4S fractions of nuclear RNA in the spleens of immunized mice.
Lipid mobilizing activity is lowered in the serum of obese patients. During short-term fasting, this activity increases in non-obese subjects. The present study was concerned with the changes in lipid mobilizing activity in the blood of obese patients during prolonged fasting, and with the behavior of esterified and free fatty acids. Serum levels of free fatty acids in the control subjects were significantly lower than in the obese women. During a 14-day period of complete fasting, levels of free fatty acids increased in all cases. In non-obese subjects this was accompanied by a significant rise in lipid mobilizing acitivity, but in obese subjects no significant change took place in this activity. The findings indicate that high levels of free fatty acids in obese persons during fasting are caused by inhibition of their esterification.
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