Wilt disease symptoms in red leaf lettuce (Lactuca sativa L.) after inoculated with Trichoderma longibrachiatum

Muhammad Iqbal Nul Hakim Mohd Sazili, F. A. Zakry, Franklin Ragai Kundat
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Abstract

Trichoderma is a fungal genus that is widely reported to cause beneficial impacts to crop plants but rarely detriment. The present study was conducted to assess the effect of inoculation of Trichoderma longibrachiatum strain UPMT14 on red leaf lettuce (Lactuca sativa L.) under growth room conditions. A culture of UPMT14 was grown on potato dextrose broth to produce inoculum. This liquid inoculum was injected through the stem of red leaf lettuce seedlings 22 days old with five replications, and then plant growth progress was monitored for vegetative responses. Red leaf lettuce plants began to exhibit foliar symptoms on day 36, such as chlorosis, wilt, and drying out, before total collapsed on day 45 in comparison to untreated control red leaf lettuce plants. In conclusion, the wilting incidence in red leaf lettuce was observed after T. longibrachiatum inoculation. Further studies are needed in future to understand the pathogenesis of T. longibrachiatum.
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红叶莴苣(Lactuca sativa L.)接种长尾木霉后的枯萎病症状
木霉是一种真菌属,被广泛报道对作物植物产生有益影响,但很少有害。在生长室内条件下,研究了接种长尾木霉UPMT14菌株对红叶莴苣的影响。在马铃薯葡萄糖肉汤上培养UPMT14以产生接种物。该液体接种剂通过22 d的红叶莴苣幼苗茎部注射,共5次重复,然后监测植株的生长过程,观察其营养反应。与未经处理的对照红叶生菜植株相比,红叶生菜植株在第36天开始表现出叶片症状,如黄化、枯萎和干燥,在第45天完全枯萎。综上所述,接种长尾曲霉后,观察到红叶莴苣的萎蔫发生率。长臂棘虫的发病机制有待进一步研究。
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