Effect of Vegetable Oils in Growth of Lactococcus garvieae Isolated from Palm Oil-contaminated Soil and Its Lipase Production

Abstract

This research aims to study the effect of vegetable oils in the growth of of Lactococcus garvieae isolated from palm oil-contaminated soil and its active lipase production. The bacteria was grown in medium containing minerals and vegetable oils (virgin olive oil, virgin coconut oil, mustard oil and sunflower oil) as sole carbon sources. The bacteria growth was measured as optical density at =600 nm. The lipase production was determined as lipase activity of crude extract toward ρ-nitrophenyl palmitate as a substrate. The results showed that the vegetable oils used provided the good growth and lipase production of L.garvieae. Even though, olive oil had a great inductive effect on bacterial productivity. The addition of 1% olive oil improved the lipase activity by 135% compared to without olive oil. Crude extract with lipase activity of 56.790 66.571 U/mL produced by L. garvieae when cultivated in medium containing 1 5% olive oil for 20 hours. Electrophoregram of SDS PAGE and zymographic analysis showed that there were 2 types of lipases produced by L. garvieae, as indicated by the presence of protein bands around 85 and 45 kDa.