首页 > 最新文献

Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)最新文献

英文 中文
Spatial Analysis in Polymerase Chain Reaction for Detection of Corynebacterium diphtheriae Post-outbreak in Samarinda 2018 聚合酶链反应检测2018年萨马林达地区白喉棒状杆菌疫情的空间分析
N. Tandirogang, G. Pratama, Yuniati, I. Fikriah, V. Abrizal, D. Bulan, Yadi
{"title":"Spatial Analysis in Polymerase Chain Reaction for Detection of Corynebacterium diphtheriae Post-outbreak in Samarinda 2018","authors":"N. Tandirogang, G. Pratama, Yuniati, I. Fikriah, V. Abrizal, D. Bulan, Yadi","doi":"10.2991/absr.k.210810.037","DOIUrl":"https://doi.org/10.2991/absr.k.210810.037","url":null,"abstract":"","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115260644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antibacterial Activity of Endophytic Fungi Isolated from Pometia pinnata J. R. Forst & G. Forst Against Escherichia coli 石榴内生真菌对大肠杆菌的抑菌活性研究
D. Prasetyani, R. Setyaningsih, A. Susilowati
The emerging of antimicrobial resistance become a global concern, encouraging researchers to find out alternative antimicrobial compounds. Endophytic fungi associated with medicinal plants become promising sources because of their ability to produce medical bioactive. This study aimed at bioprospecting fungal endophytes from an ethnobotanical medicinal plant, Pometia pinnata J. R. Forst & G Forst for their antimicrobial activity. Endophytic fungi isolated from leaf, stem, and root aseptically. Ten selected endophytes were cultured and then extracted using ethyl acetate. Screening antibacterial activity of ethyl acetate extract carried out by paper disk diffusion assay against Escherichia coli ATCC 25922. TLC-bioautography assay was used to study the type of secondary metabolite which has antibacterial activity. Identification of endophytic fungi based on morphology and ITS sequence using ITS1 and ITS4 primers. The result showed that two isolates have antibacterial activity but only one isolate exhibits strong antibacterial activity with a diameter of inhibition zone 21 mm. The isolate was identified as Aspergillus minisclerotigenes with 100% identity of the ITS sequence using BLAST software on NCBI. TLC assay indicated that ethyl acetate extract of A. minisclerotigenes contains alkaloids, phenols, flavonoids, and terpenoids while TLC bioautography showed that its phenolic compound appeared antibacterial activity.
抗菌素耐药性的出现成为全球关注的问题,鼓励研究人员寻找替代抗菌素化合物。与药用植物相关的内生真菌因其具有产生药用生物活性的能力而成为很有前景的资源。本研究旨在对民族植物药用植物石榴(pomtia pinnata J. R. Forst & G . Forst)内生真菌的抑菌活性进行生物勘探。从叶、茎和根中无菌分离的内生真菌。选择10株内生菌,用乙酸乙酯提取。采用纸片扩散法筛选乙酸乙酯提取物对大肠杆菌ATCC 25922的抑菌活性。采用tlc -生物自显影法研究了具有抗菌活性的次生代谢物类型。利用ITS1和ITS4引物基于形态学和ITS序列的内生真菌鉴定。结果表明,2株菌株均有抑菌活性,但只有1株具有较强抑菌活性,抑菌带直径为21 mm。利用NCBI上的BLAST软件鉴定该分离物为微绿曲霉(Aspergillus mincleerotigenes), ITS序列同源性100%。薄层色谱分析结果表明,细刺草乙酸乙酯提取物中含有生物碱、酚类、黄酮类和萜类化合物,薄层生物图谱显示其酚类化合物具有抗菌活性。
{"title":"Antibacterial Activity of Endophytic Fungi Isolated from Pometia pinnata J. R. Forst & G. Forst Against Escherichia coli","authors":"D. Prasetyani, R. Setyaningsih, A. Susilowati","doi":"10.2991/absr.k.210810.024","DOIUrl":"https://doi.org/10.2991/absr.k.210810.024","url":null,"abstract":"The emerging of antimicrobial resistance become a global concern, encouraging researchers to find out alternative antimicrobial compounds. Endophytic fungi associated with medicinal plants become promising sources because of their ability to produce medical bioactive. This study aimed at bioprospecting fungal endophytes from an ethnobotanical medicinal plant, Pometia pinnata J. R. Forst & G Forst for their antimicrobial activity. Endophytic fungi isolated from leaf, stem, and root aseptically. Ten selected endophytes were cultured and then extracted using ethyl acetate. Screening antibacterial activity of ethyl acetate extract carried out by paper disk diffusion assay against Escherichia coli ATCC 25922. TLC-bioautography assay was used to study the type of secondary metabolite which has antibacterial activity. Identification of endophytic fungi based on morphology and ITS sequence using ITS1 and ITS4 primers. The result showed that two isolates have antibacterial activity but only one isolate exhibits strong antibacterial activity with a diameter of inhibition zone 21 mm. The isolate was identified as Aspergillus minisclerotigenes with 100% identity of the ITS sequence using BLAST software on NCBI. TLC assay indicated that ethyl acetate extract of A. minisclerotigenes contains alkaloids, phenols, flavonoids, and terpenoids while TLC bioautography showed that its phenolic compound appeared antibacterial activity.","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115136196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabolome profile of osmotolerant rhizobacteria under osmotic stress 渗透胁迫下耐渗透根瘤菌的代谢组谱
T. A. Surya, O. Palupi, T. Yuwono, Ngadiman, S. Wedhastri
An osmotolerant rhizobacteria (Enterococcus flavescens), isolated from the weed rhizosphere, has been subjected to osmotic stress in broth media supplemented with varied concentrations of glucose and sodium chloride (NaCl). The aims of this work was to evaluate the cells’ response and obtain a comprehensive view of metabolites profiles under osmotic stress. Cells were grown in rich medium (Luria Bertani, LB) supplemented with NaCl at varying concentrations (0.5 M – 1.8 M NaCl) and glucose (5% – 20%), both as a single or double stress condition. Physiological response was observed by measuring rhizobacterial growth and analysing metabolite synthesised using GC-MS. The results of this study demonstrated that different concentration of salt and glucose resulted in different metabolite profiles. It was also observed that cells cultivated in LB + 1,6 M NaCl and LB + 1,8 M NaCl medium resulted in only two different compounds detected, i.e amide and fatty acid. Under double stress, however, concentration of hexadecanoic acid, hexadecenoic acid, proline and glycerin was found increased, while under single-salt and glucose stress, hexadecanoic acid and 9octadecenoic acidmethyl ester were detected. Those two acid were absent from the cells’ metabolites under double stress. The results of this study thus suggested that osmotic stress provokes different pathway of fatty acid metabolism. The metabolome profile of the rhizobacteria under stress condition may also reveal specific mechanism of microbial tolerance to osmotic stress under different condition. The possible mechanism and pathway of osmotic stress in microorganisms will be discussed.
从杂草根际分离出的一种耐渗透根瘤菌(黄肠球菌)在添加不同浓度葡萄糖和氯化钠(NaCl)的肉汤培养基中受到渗透胁迫。这项工作的目的是评估细胞的反应,并获得在渗透胁迫下代谢物谱的全面视图。细胞在富培养基(Luria Bertani, LB)中生长,培养基中添加不同浓度的NaCl (0.5 M - 1.8 M NaCl)和葡萄糖(5% - 20%),分别作为单一或双重胁迫条件。采用气相色谱-质谱法分析合成的代谢物和根细菌的生长情况,观察其生理反应。本研究结果表明,不同浓度的盐和葡萄糖会导致不同的代谢产物谱。在LB + 1,6 M NaCl和LB + 1,8 M NaCl培养基中培养的细胞只检测到两种不同的化合物,即酰胺和脂肪酸。双盐胁迫下,16酸、16烯酸、脯氨酸和甘油浓度升高,单盐和葡萄糖胁迫下,16酸和90十八烯酸甲酯浓度升高。在双重胁迫下,细胞代谢产物中没有这两种酸。本研究结果提示,渗透胁迫引发了不同的脂肪酸代谢途径。胁迫条件下的根菌代谢组谱也可能揭示不同条件下微生物对渗透胁迫耐受的特定机制。本文将讨论微生物渗透胁迫的可能机制和途径。
{"title":"Metabolome profile of osmotolerant rhizobacteria under osmotic stress","authors":"T. A. Surya, O. Palupi, T. Yuwono, Ngadiman, S. Wedhastri","doi":"10.2991/absr.k.210810.036","DOIUrl":"https://doi.org/10.2991/absr.k.210810.036","url":null,"abstract":"An osmotolerant rhizobacteria (Enterococcus flavescens), isolated from the weed rhizosphere, has been subjected to osmotic stress in broth media supplemented with varied concentrations of glucose and sodium chloride (NaCl). The aims of this work was to evaluate the cells’ response and obtain a comprehensive view of metabolites profiles under osmotic stress. Cells were grown in rich medium (Luria Bertani, LB) supplemented with NaCl at varying concentrations (0.5 M – 1.8 M NaCl) and glucose (5% – 20%), both as a single or double stress condition. Physiological response was observed by measuring rhizobacterial growth and analysing metabolite synthesised using GC-MS. The results of this study demonstrated that different concentration of salt and glucose resulted in different metabolite profiles. It was also observed that cells cultivated in LB + 1,6 M NaCl and LB + 1,8 M NaCl medium resulted in only two different compounds detected, i.e amide and fatty acid. Under double stress, however, concentration of hexadecanoic acid, hexadecenoic acid, proline and glycerin was found increased, while under single-salt and glucose stress, hexadecanoic acid and 9octadecenoic acidmethyl ester were detected. Those two acid were absent from the cells’ metabolites under double stress. The results of this study thus suggested that osmotic stress provokes different pathway of fatty acid metabolism. The metabolome profile of the rhizobacteria under stress condition may also reveal specific mechanism of microbial tolerance to osmotic stress under different condition. The possible mechanism and pathway of osmotic stress in microorganisms will be discussed.","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116925877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lactic Acid Bacteria Fermentation of High Protein Feeds: The Effect of Molasses and Incubation Time on Improving Digestibility 高蛋白饲料乳酸菌发酵:糖蜜和发酵时间对提高消化率的影响
P. G. Kinayang, Z. Bachruddin, A. Kurniawati
{"title":"Lactic Acid Bacteria Fermentation of High Protein Feeds: The Effect of Molasses and Incubation Time on Improving Digestibility","authors":"P. G. Kinayang, Z. Bachruddin, A. Kurniawati","doi":"10.2991/absr.k.210810.029","DOIUrl":"https://doi.org/10.2991/absr.k.210810.029","url":null,"abstract":"","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125025260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation and Identification of Bacteriocin-producing Bacillus Strain Isolated from the Gastrointestinal Tract of Indonesian Native Chicken (Gallus domesticus) 印尼家鸡胃肠道产菌素芽孢杆菌的分离与鉴定
C. Hanim, V. A. Cahya, L. M. Yusiati, A. Kurniawati
This research aimed to obtain Bacillus bacteria from the gastrointestinal tract (GIT) of native Indonesian chicken (Gallus domesticus) for bacteriocin production purposes against Escherichia coli. Bacillus bacteria were isolated from GIT (duodenum, jejunum, and ileum) on Trypticase Soya Agar (TSA+5% NaCl). The dilution was heated at 80°C for 20 min to kill other bacteria and induced Bacillus spore. Screening method based on the ability to against pathogens, then microbiological and biochemical characteristics. Inhibition test against pathogen using a well diffuse method. Thirteen Bacillus isolates then continued for testing against Escherichia coli FNCC 0091 for bacteriocin production purposes. Biochemical identification using conventional identification. The selected Bacillus bacteria, which had the highest inhibition zone, were isolates 2, 3B, and 11A (23.7, 18.6, and 19.9 mm). Biochemical and microbiological identification revealed that the isolate 2 isolated from duodenum was mixed culture (Bacillus subtilis, and Bacillus sp.), isolate 3B isolated from jejunum was mixed culture (Bacillus subtilis, Bacillus sp., and Bacillus sp.) and isolate 11A isolated from ileum was pure culture (Bacillus subtilis). All isolates were identified as rod-shaped, Gram-positive, aerobic, endospore-forming bacteria, and produced catalase.
本研究旨在从印尼本土鸡(Gallus domesticus)的胃肠道(GIT)中获得芽孢杆菌,用于生产对抗大肠杆菌的细菌素。在胰酶大豆琼脂(TSA+5% NaCl)培养基上,从胃肠道(十二指肠、空肠和回肠)分离到芽孢杆菌。稀释液在80℃下加热20 min,杀死其他细菌并诱导芽孢杆菌孢子。筛选方法基于对病原体的能力,然后是微生物学和生化特性。采用良好扩散法对病原菌进行抑制试验。随后,13株芽孢杆菌分离株继续对大肠杆菌FNCC 0091进行细菌素生产试验。生化鉴定采用常规鉴定。结果表明,菌株2、3B和11A(23.7、18.6和19.9 mm)的抑菌带最高。生化和微生物学鉴定表明,从十二指肠分离的分离物2为混合培养(枯草芽孢杆菌和芽孢杆菌),从空肠分离的分离物3B为混合培养(枯草芽孢杆菌、芽孢杆菌和芽孢杆菌),从回肠分离的分离物11A为纯培养(枯草芽孢杆菌)。所有分离株均为杆状,革兰氏阳性,需氧,内孢子形成细菌,并产生过氧化氢酶。
{"title":"Isolation and Identification of Bacteriocin-producing Bacillus Strain Isolated from the Gastrointestinal Tract of Indonesian Native Chicken (Gallus domesticus)","authors":"C. Hanim, V. A. Cahya, L. M. Yusiati, A. Kurniawati","doi":"10.2991/absr.k.210810.007","DOIUrl":"https://doi.org/10.2991/absr.k.210810.007","url":null,"abstract":"This research aimed to obtain Bacillus bacteria from the gastrointestinal tract (GIT) of native Indonesian chicken (Gallus domesticus) for bacteriocin production purposes against Escherichia coli. Bacillus bacteria were isolated from GIT (duodenum, jejunum, and ileum) on Trypticase Soya Agar (TSA+5% NaCl). The dilution was heated at 80°C for 20 min to kill other bacteria and induced Bacillus spore. Screening method based on the ability to against pathogens, then microbiological and biochemical characteristics. Inhibition test against pathogen using a well diffuse method. Thirteen Bacillus isolates then continued for testing against Escherichia coli FNCC 0091 for bacteriocin production purposes. Biochemical identification using conventional identification. The selected Bacillus bacteria, which had the highest inhibition zone, were isolates 2, 3B, and 11A (23.7, 18.6, and 19.9 mm). Biochemical and microbiological identification revealed that the isolate 2 isolated from duodenum was mixed culture (Bacillus subtilis, and Bacillus sp.), isolate 3B isolated from jejunum was mixed culture (Bacillus subtilis, Bacillus sp., and Bacillus sp.) and isolate 11A isolated from ileum was pure culture (Bacillus subtilis). All isolates were identified as rod-shaped, Gram-positive, aerobic, endospore-forming bacteria, and produced catalase.","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116075601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Xylanase Purification of Indigenous Aspergillus tamari TKM-24 Isolated from Environmental Soil in Borneo Island 婆罗洲岛环境土壤土生柽柳曲霉TKM-24木聚糖酶的纯化
S. Margino, Y. Mallisa, W. Asmara
{"title":"Xylanase Purification of Indigenous Aspergillus tamari TKM-24 Isolated from Environmental Soil in Borneo Island","authors":"S. Margino, Y. Mallisa, W. Asmara","doi":"10.2991/absr.k.210810.040","DOIUrl":"https://doi.org/10.2991/absr.k.210810.040","url":null,"abstract":"","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114993698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Potential Yeast Strains for Biological Control of Mosquitoes 一种可能用于蚊虫生物防治的酵母菌株
D. Satwika, V. Permatasari, G. E. N. Cahyani
{"title":"A Potential Yeast Strains for Biological Control of Mosquitoes","authors":"D. Satwika, V. Permatasari, G. E. N. Cahyani","doi":"10.2991/absr.k.210810.023","DOIUrl":"https://doi.org/10.2991/absr.k.210810.023","url":null,"abstract":"","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127278619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation and Characterization of Wild Type Acetobacter xylinum from Nata de Coco Industry in Surakarta Residency 苏拉塔地区椰子树工业野生型木醋杆菌的分离与鉴定
E. R. Karuni, A. Sari, A. Nursiwi, A. Sanjaya
{"title":"Isolation and Characterization of Wild Type Acetobacter xylinum from Nata de Coco Industry in Surakarta Residency","authors":"E. R. Karuni, A. Sari, A. Nursiwi, A. Sanjaya","doi":"10.2991/absr.k.210810.009","DOIUrl":"https://doi.org/10.2991/absr.k.210810.009","url":null,"abstract":"","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"144 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129058027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
The Role of Synbiotic in Cobb-strain Broiler Performance Challenged with Campylobacter jejuni as a Substitute for Antibiotic Growth Promotor (AGP) 合成菌替代抗生素生长促进剂(AGP)对cobb型肉鸡生产性能的影响
A. Wahyuni, T. Nahak, M. C. C. Malelak, V. C. Prakasita, S. L. Adrenalin
One of the feed additives that have long been used is Antibiotic Growth Promoter (AGP). But nowadays, the use of AGP as a growth promoter has been banned because it has negative impacts such as antimicrobial resistance. Campylobacter jejuni is a pathogenic bacterium that often infects birds, especially broilers. This research aims to investigate the role of synbiotic composed of a prebiotic, and Saccharomyces sp. and Lactobacillus sp. as probiotics (commercial products) in performance of broilers challenged with C. jejuni as a substitute for AGP. Twenty-one Day Old Chicks (DOC) of Cobb-strain broilers were divided into three groups, each consisting of 7 chicks. Group I was given basal diets, group II was given basal diets and AGP (with 250 g/ton of enramycin), and group III was given basal diets and synbiotic (100 gram/100 kg). C. jejuni challenge test was carried out at the 3 week. The results suggested that at week 4, group III showed more and significantly different body weight from that of group I, but it did not differ significantly from that of group II. Similarly, at week 4 the weight gain of group III was higher and significantly different compared to that of group I, but did not differ significantly from that of group II. Also, the carcass percentage at week 5 did not differ significantly among the groups, but group III had a higher percentage of carcasses than groups I and II. The addition of synbiotic can replace AGP as a feed additive because it can improve the performance of broilers challenged by C. jejuni.
抗生素生长促进剂(AGP)是长期使用的饲料添加剂之一。但是现在,AGP作为生长促进剂的使用已经被禁止,因为它有诸如抗菌素耐药性等负面影响。空肠弯曲杆菌是一种常感染禽类,尤其是肉鸡的致病菌。本研究旨在探讨由益生元组成的益生菌,以及以酵母菌和乳杆菌为益生菌(商品)的益生菌对空肠梭菌替代AGP攻毒肉鸡生产性能的影响。将21日龄柯布系肉鸡(DOC)分为3组,每组7只。ⅰ组饲喂基础饲粮,ⅱ组饲喂基础饲粮+ AGP(添加250 g/t enramycin),ⅲ组饲喂基础饲粮+合成饲粮(100 g/ 100 kg)。3周时进行空肠梭菌攻毒试验。结果表明,在第4周时,III组的体重与I组相比差异更大且显著,但与II组差异不显著。同样,第4周时,III组的增重高于I组,且与I组有显著差异,但与II组无显著差异。第5周胴体率各组间差异不显著,但ⅲ组胴体率高于ⅰ组和ⅱ组。在肉鸡饲粮中添加合成菌可以提高空肠梭菌侵毒肉鸡的生产性能,可以替代AGP作为饲料添加剂。
{"title":"The Role of Synbiotic in Cobb-strain Broiler Performance Challenged with Campylobacter jejuni as a Substitute for Antibiotic Growth Promotor (AGP)","authors":"A. Wahyuni, T. Nahak, M. C. C. Malelak, V. C. Prakasita, S. L. Adrenalin","doi":"10.2991/absr.k.210810.001","DOIUrl":"https://doi.org/10.2991/absr.k.210810.001","url":null,"abstract":"One of the feed additives that have long been used is Antibiotic Growth Promoter (AGP). But nowadays, the use of AGP as a growth promoter has been banned because it has negative impacts such as antimicrobial resistance. Campylobacter jejuni is a pathogenic bacterium that often infects birds, especially broilers. This research aims to investigate the role of synbiotic composed of a prebiotic, and Saccharomyces sp. and Lactobacillus sp. as probiotics (commercial products) in performance of broilers challenged with C. jejuni as a substitute for AGP. Twenty-one Day Old Chicks (DOC) of Cobb-strain broilers were divided into three groups, each consisting of 7 chicks. Group I was given basal diets, group II was given basal diets and AGP (with 250 g/ton of enramycin), and group III was given basal diets and synbiotic (100 gram/100 kg). C. jejuni challenge test was carried out at the 3 week. The results suggested that at week 4, group III showed more and significantly different body weight from that of group I, but it did not differ significantly from that of group II. Similarly, at week 4 the weight gain of group III was higher and significantly different compared to that of group I, but did not differ significantly from that of group II. Also, the carcass percentage at week 5 did not differ significantly among the groups, but group III had a higher percentage of carcasses than groups I and II. The addition of synbiotic can replace AGP as a feed additive because it can improve the performance of broilers challenged by C. jejuni.","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127618887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Role of Probiotics in Suppressing Campylobacter jejuni Infection in Broilers: Performance Parameters 益生菌抑制肉鸡空肠弯曲杆菌感染的作用:性能参数
T. Nahak, V. C. Prakasita, L. N. Imanjati, S. L. Adrenalin, C. R. Tabbu, A. Wahyuni
{"title":"The Role of Probiotics in Suppressing Campylobacter jejuni Infection in Broilers: Performance Parameters","authors":"T. Nahak, V. C. Prakasita, L. N. Imanjati, S. L. Adrenalin, C. R. Tabbu, A. Wahyuni","doi":"10.2991/absr.k.210810.019","DOIUrl":"https://doi.org/10.2991/absr.k.210810.019","url":null,"abstract":"","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132542952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1